{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:21:45Z","timestamp":1791415305663,"version":"4.3.3"},"reference-count":72,"publisher":"American Physiological Society","issue":"2","funder":[{"DOI":"10.13039\/100011781","name":"Shriners Hospital for Children","doi-asserted-by":"crossref","award":["80100"],"award-info":[{"award-number":["80100"]}],"id":[{"id":"10.13039\/100011781","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100011781","name":"Shriners Hospital for Children","doi-asserted-by":"crossref","award":["85310"],"award-info":[{"award-number":["85310"]}],"id":[{"id":"10.13039\/100011781","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100011781","name":"Shriners Hospital for Children","doi-asserted-by":"crossref","award":["84090"],"award-info":[{"award-number":["84090"]}],"id":[{"id":"10.13039\/100011781","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100011781","name":"Shriners Hospital for Children","doi-asserted-by":"crossref","award":["84080"],"award-info":[{"award-number":["84080"]}],"id":[{"id":"10.13039\/100011781","id-type":"DOI","asserted-by":"crossref"}]},{"name":"NIH","award":["RO1 AR049877"],"award-info":[{"award-number":["RO1 AR049877"]}]},{"name":"NIH","award":["RO1 GM56687"],"award-info":[{"award-number":["RO1 GM56687"]}]},{"name":"NIH","award":["P50 GM060388"],"award-info":[{"award-number":["P50 GM060388"]}]},{"name":"NIH","award":["UL1 TR000071"],"award-info":[{"award-number":["UL1 TR000071"]}]},{"name":"NIH","award":["T32-GM8256"],"award-info":[{"award-number":["T32-GM8256"]}]},{"name":"Department of Surgery at University of Texas Medical Branch at Galveston"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2016,8,1]]},"abstract":"<jats:p>Burn trauma results in prolonged hypermetabolism and skeletal muscle wasting. How hypermetabolism contributes to muscle wasting in burn patients remains unknown. We hypothesized that oxidative stress, cytosolic protein degradation, and mitochondrial stress as a result of hypermetabolism contribute to muscle cachexia postburn. Patients ( n = 14) with burns covering &gt;30% of their total body surface area were studied. Controls ( n = 13) were young healthy adults. We found that burn patients were profoundly hypermetabolic at both the skeletal muscle and systemic levels, indicating increased oxygen consumption by mitochondria. In skeletal muscle of burn patients, concurrent activation of mTORC1 signaling and elevation in the fractional synthetic rate paralleled increased levels of proteasomes and elevated fractional breakdown rate. Burn patients had greater levels of oxidative stress markers as well as higher expression of mtUPR-related genes and proteins, suggesting that burns increased mitochondrial stress and protein damage. Indeed, upregulation of cytoprotective genes suggests hypermetabolism-induced oxidative stress postburn. In parallel to mtUPR activation postburn, mitochondrial-specific proteases (LONP1 and CLPP) and mitochondrial translocases (TIM23, TIM17B, and TOM40) were upregulated, suggesting increased mitochondrial protein degradation and transport of preprotein, respectively. Our data demonstrate that proteolysis occurs in both the cytosolic and mitochondrial compartments of skeletal muscle in severely burned patients. Increased mitochondrial protein turnover may be associated with increased protein damage due to hypermetabolism-induced oxidative stress and activation of mtUPR. Our results suggest a novel role for the mitochondria in burn-induced cachexia.<\/jats:p>","DOI":"10.1152\/ajpendo.00535.2015","type":"journal-article","created":{"date-parts":[[2016,7,6]],"date-time":"2016-07-06T02:19:29Z","timestamp":1467771569000},"page":"E436-E448","source":"Crossref","is-referenced-by-count":49,"title":["Hypermetabolism and hypercatabolism of skeletal muscle accompany mitochondrial stress following severe burn trauma"],"prefix":"10.1152","volume":"311","author":[{"given":"John O.","family":"Ogunbileje","sequence":"first","affiliation":[{"name":"Metabolism Unit, Shriners Hospitals for Children, Galveston, Texas;"},{"name":"Department of Surgery, University of Texas Medical Branch, Galveston, Texas;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Porter","sequence":"additional","affiliation":[{"name":"Metabolism Unit, Shriners Hospitals for Children, Galveston, Texas;"},{"name":"Department of Surgery, University of Texas Medical Branch, Galveston, Texas;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David N.","family":"Herndon","sequence":"additional","affiliation":[{"name":"Metabolism Unit, Shriners Hospitals for Children, Galveston, Texas;"},{"name":"Department of Surgery, University of Texas Medical Branch, Galveston, Texas;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony","family":"Chao","sequence":"additional","affiliation":[{"name":"Metabolism Unit, Shriners Hospitals for Children, Galveston, Texas;"},{"name":"Department of Surgery, University of Texas Medical Branch, Galveston, Texas;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doaa R.","family":"Abdelrahman","sequence":"additional","affiliation":[{"name":"Metabolism Unit, Shriners Hospitals for Children, Galveston, Texas;"},{"name":"Department of Surgery, University of Texas Medical Branch, Galveston, Texas;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Papadimitriou","sequence":"additional","affiliation":[{"name":"Metabolism Unit, Shriners Hospitals for Children, Galveston, Texas;"},{"name":"Department of Nutrition and Metabolism, University of Texas Medical Branch, Galveston, Texas;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Chondronikola","sequence":"additional","affiliation":[{"name":"Metabolism Unit, Shriners Hospitals for Children, Galveston, Texas;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa A.","family":"Zimmers","sequence":"additional","affiliation":[{"name":"Department of Surgery, Indiana University School of Medicine, Indianapolis, Indiana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul T.","family":"Reidy","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Metabolism, University of Texas Medical Branch, Galveston, Texas;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blake B.","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Metabolism, University of Texas Medical Branch, Galveston, Texas;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Labros S.","family":"Sidossis","sequence":"additional","affiliation":[{"name":"Metabolism Unit, Shriners Hospitals for Children, Galveston, Texas;"},{"name":"Department of Surgery, University of Texas Medical Branch, Galveston, Texas;"},{"name":"Department of Kinesiology and Health, Rutgers University, New Brunswick, New Jersey; and"},{"name":"Robert Wood Johnson Medical School, Rutgers University, New Brunswick, New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20150436"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1042\/bse0410173"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2015.00040"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2011.01.004"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.106.061580"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.3109\/00365517509095787"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.87.7.8699"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1038\/ncb836"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(02)03638-4"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2007.11.003"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007095"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000000454"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.300559"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000000075"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80428-6"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00600.2013"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.363614"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1067\/msy.2000.108059"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1067\/msy.2000.108059"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201500013"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3976"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01270-12"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2013.12.029"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.21882"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e31823e76e7"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e318146980e"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.090738"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1002\/iub.114"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2013.01.004"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2013.12.008"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2443.2007.01099.x"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.10.041"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200708199"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.078089-0"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2006010083"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12350"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1019209108"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2013.03.022"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1097\/BCR.0b013e3181d0f55d"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2007.04.004"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-014-9525-z"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20081386"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2013.01.015"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2012.02.019"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1038\/35073006"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1997.273.1.E99"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1097\/TA.0b013e3182921651"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2015.10.026"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000000410"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1097\/BCR.0000000000000308"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00206.2014"},{"key":"B52","first-page":"9","volume":"3","author":"Porter C","year":"2013","journal-title":"Int J Burns Trauma"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.01856"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0307446101"},{"key":"B55","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1002\/em.20553","volume":"51","author":"Roede JR","year":"2010","journal-title":"Environ Mol Mutagen"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/40.1.168"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060408-172623"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2015.01.002"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2014.06.004"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2015.06.022"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1042\/BST0391509"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.049478"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2009.2998"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.19.14064"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1949.sp004363"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R400003200"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.1016\/j.cps.2009.05.001"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1177\/014860718000400215"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2011.07.001"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00053.2002"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf445"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M409034200"}],"container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpendo.00535.2015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T03:45:35Z","timestamp":1718682335000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpendo.00535.2015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,1]]},"references-count":72,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,8,1]]}},"alternative-id":["10.1152\/ajpendo.00535.2015"],"URL":"https:\/\/doi.org\/10.1152\/ajpendo.00535.2015","relation":{},"ISSN":["0193-1849","1522-1555"],"issn-type":[{"value":"0193-1849","type":"print"},{"value":"1522-1555","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,1]]}}}