{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T16:06:47Z","timestamp":1764259607361},"reference-count":39,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2008,2]]},"abstract":"Previous studies have demonstrated that macrophage-derived apolipoprotein E (apoE) reduces atherosclerotic lesion formation in lean apoE-deficient (\u2212\/\u2212<\/jats:sup>) mice. apoE has also been demonstrated to play a role in adipocyte differentiation and lipid accumulation. Because the prevalence of obesity has grown to epidemic proportions, we sought to determine whether macrophage-derived apoE could impact atherosclerotic lesion formation or adipose tissue expansion and inflammation in obese apoE\u2212\/\u2212<\/jats:sup>mice. To this end, we transplanted obese leptin-deficient ( ob\/ ob) apoE\u2212\/\u2212<\/jats:sup>mice with bone marrow from either ob\/ ob;apoE\u2212\/\u2212<\/jats:sup>or ob\/ ob;apoE+\/+<\/jats:sup>donors. There were no differences in body weight, total body adipose tissue, or visceral fat pad mass between recipient groups. The presence of macrophage-apoE had no impact on adipose tissue macrophage content or inflammatory cytokine expression. Recipients of apoE+\/+<\/jats:sup>marrow demonstrated 3.7-fold lower plasma cholesterol ( P < 0.001) and 1.7-fold lower plasma triglyceride levels ( P < 0.01) by 12 wk after transplantation even though apoE was present in plasma at concentrations <10% of wild-type levels. The reduced plasma lipids reflected a dramatic decrease in very low density lipoprotein and a mild increase in high-density lipoprotein levels. Atherosclerotic lesion area was >10-fold lower in recipients of ob\/ ob;apoE+\/+<\/jats:sup>marrow ( P < 0.005). Similar results were seen in leptin receptor-deficient ( db\/ db) apoE\u2212\/\u2212<\/jats:sup>mice. Finally, when bone marrow transplantation was performed in 4-mo-old ob\/ ob;apoE\u2212\/\u2212<\/jats:sup>and db\/ db;apoE\u2212\/\u2212<\/jats:sup>mice with preexisting lesions, recipients of apoE+\/+<\/jats:sup>marrow had a 2.8-fold lower lesion area than controls ( P = 0.0002). These results demonstrate that macrophage-derived apoE does not impact adipose tissue expansion or inflammatory status; however, even very low levels of macrophage-derived apoE are capable of reducing plasma lipids and atherosclerotic lesion area in obese mice.<\/jats:p>","DOI":"10.1152\/ajpendo.00601.2007","type":"journal-article","created":{"date-parts":[[2007,11,21]],"date-time":"2007-11-21T01:44:44Z","timestamp":1195609484000},"page":"E284-E290","source":"Crossref","is-referenced-by-count":15,"title":["Macrophage-derived apolipoprotein E ameliorates dyslipidemia and atherosclerosis in obese apolipoprotein E-deficient mice"],"prefix":"10.1152","volume":"294","author":[{"given":"Robin D.","family":"Atkinson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly R.","family":"Coenen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle 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