{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T05:57:12Z","timestamp":1773295032154,"version":"3.50.1"},"reference-count":24,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:p>The presence of carbonic anhydrase (type V) was recently documented in rat and mouse pancreatic islet \u03b2-cells by immunostaining and Western blotting. In the present study, the activity of carbonic anhydrase was measured in rat islet homogenates and shown to be about four times lower than in rat parotid cells. The pattern for the inhibitory action of acetazolamide on carbonic anhydrase activity also differed in islet and parotid cell homogenates, suggesting the presence of different isoenzymes. NaN<jats:sub>3<\/jats:sub>inhibited carbonic anhydrase activity in islet homogenates and both d-[U-<jats:sup>14<\/jats:sup>C]glucose oxidation and glucose-stimulated insulin secretion. Acetazolamide (0.3\u201310.0 mM) also decreased glucose-induced insulin output but failed to affect adversely d-[U-<jats:sup>14<\/jats:sup>C]glucose oxidation, although it inhibited the conversion of d-[5-<jats:sup>3<\/jats:sup>H]glucose to [<jats:sup>3<\/jats:sup>H]OH and that of d-[U-<jats:sup>14<\/jats:sup>C]glucose to acidic metabolites. Hydrochlorothiazide (3.0\u201310.0 mM), which also caused a concentration-related inhibition of the secretory response, like acetazolamide (5.0\u201310.0 mM), decreased H<jats:sup>14<\/jats:sup>CO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>production from d-[U-<jats:sup>14<\/jats:sup>C]glucose (16.7 mM). Acetazolamide (5.0 mM) did not affect the activity of volume-sensitive anion channels in \u03b2-cells but lowered intracellular pH and adversely affected both the bioelectrical response to d-glucose and its effect on the cytosolic concentration of Ca<jats:sup>2+<\/jats:sup>in these cells. The lowering of cellular pH by acetazolamide, which could well be due to inhibition of carbonic anhydrase, might in turn account for inhibition of glycolysis. The perturbation of stimulus-secretion coupling in the \u03b2-cells exposed to acetazolamide may thus involve impaired circulation in the pyruvate-malate shuttle, altered mitochondrial Ca<jats:sup>2+<\/jats:sup>accumulation, and perturbation of Cl<jats:sup>\u2212<\/jats:sup>fluxes, resulting in both decreased bioelectrical activity and insulin release.<\/jats:p>","DOI":"10.1152\/ajpendo.00631.2006","type":"journal-article","created":{"date-parts":[[2007,2,7]],"date-time":"2007-02-07T08:57:51Z","timestamp":1170838671000},"page":"E1624-E1630","source":"Crossref","is-referenced-by-count":20,"title":["Possible role of carbonic anhydrase in rat pancreatic islets: enzymatic, secretory, metabolic, ionic, and electrical aspects"],"prefix":"10.1152","volume":"292","author":[{"given":"Abdullah","family":"Sener","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hassan","family":"Jijakli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saleh Zahedi","family":"Asl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Courtois","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allen P.","family":"Yates","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvain","family":"Meuris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonard C.","family":"Best","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Willy J.","family":"Malaisse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","unstructured":"Berliner RW, Orloff J.Carbonic anhydrase inhibitors.Pharmacol Rev8: 137\u2013174, 1956."},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1007\/s001250050635"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/S0898-6568(01)00223-6"},{"key":"R4","unstructured":"Best L.How does glucose evoke electrical activity in the pancreatic beta-cell ?Diabetes Metab26: 3S18\u20133S24, 2002."},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(95)03568-R"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(83)80882-5"},{"key":"R7","unstructured":"Jijakli H, Zhang H, Sener A, Malaisse WJ.Properties of pancreatic islet beta cells obtained by immunomagnetic purification (Abstract).Diabetologia45: A139, 2002."},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119071"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/S1056-8719(97)00095-6"},{"key":"R10","doi-asserted-by":"crossref","unstructured":"Lowry O, Rosebrough NJ, Farr AL, Randall RJ.Protein measurement by the Folin phenol reagent.J Biol Chem193: 265\u2013275, 1951.","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"R11","doi-asserted-by":"crossref","unstructured":"MacDonald MJ.Feasibility of a mitochondrial pyruvate malate shuttle in pancreatic islets. 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