{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T14:08:44Z","timestamp":1790431724486,"version":"4.1.0"},"reference-count":40,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:p> In the present study, we investigated the ability of human fetal membranes (amnion and choriodecidua) to regulate human maternal uterine cell functions through the secretion of surfactant protein (SP)-A and SP-D at the end of pregnancy. We detected the expression of both SP-A (SP-A1 and SP-A2) and SP-D by quantitative reverse transcription polymerase chain reaction. Immunohistochemistry revealed that human fetal membranes expressed both SP-A and SP-D. By Western blot analysis, we demonstrated that SP-A protein expression was predominant in choriodecidua, whereas the amnion predominantly expressed SP-D. Only the secretion of SP-A was evidenced in the culture supernatants of amnion and choriodecidua explants by immunodot blot and confirmed by Western blot. Exogenous human purified SP-A induced stress fiber formation in cultured human myometrial cells via a pathway involving Rho-kinase. Conditioned medium from choriodecidua and amnion explants mimicked the SP-A effect. Treatment of myometrial cells with SP-A-depleted conditioned medium from choriodecidua or amnion explants failed to change the actin dynamic. These data indicate that SP-A released by human fetal membranes is able to exert a paracrine regulation of F-actin filament organization in myometrial cells. <\/jats:p>","DOI":"10.1152\/ajpendo.00746.2009","type":"journal-article","created":{"date-parts":[[2010,3,17]],"date-time":"2010-03-17T01:47:18Z","timestamp":1268790438000},"page":"E1188-E1197","source":"Crossref","is-referenced-by-count":13,"title":["Secreted surfactant protein A from fetal membranes induces stress fibers in cultured human myometrial cells"],"prefix":"10.1152","volume":"298","author":[{"given":"Michelle","family":"Breuiller-Fouch\u00e9","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), U767, Facult\u00e9 des Sciences Pharmaceutiques et Biologiques, Universit\u00e9 Paris Descartes Paris V, 75006, Paris;"},{"name":"PremUp, Paris, 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