{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:11:32Z","timestamp":1773418292400,"version":"3.50.1"},"reference-count":39,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:p> Uncoupling protein-3 (UCP3) is a mitochondrial inner-membrane protein highly expressed in skeletal muscle. While UCP3's function is still unknown, it has been hypothesized to act as a fatty acid (FA) anion exporter, protecting mitochondria against lipid peroxidation and\/or facilitating FA oxidation. The aim of this study was to determine the effects of long-term feeding of a 45% fat diet on whole body indicators of muscle metabolism in congenic C57BL\/6 mice that were either lacking UCP3 ( Ucp3<jats:sup>\u2212\/\u2212<\/jats:sup>) or had a transgenically induced approximately twofold increase in UCP3 levels ( UCP3tg). Mice were fed the high-fat (HF) diet for a period of either 4 or 8 mo immediately following weaning. After long-term HF feeding, UCP3tg mice weighed an average of 15% less than wild-type mice ( P &lt; 0.05) and were 20% less metabolically efficient than both wild-type and Ucp3<jats:sup>\u2212\/\u2212<\/jats:sup> mice ( P &lt; 0.01). Additionally, wild-type mice had 21% lower, whereas UCP3tg mice had 36% lower, levels of adiposity compared with Ucp3<jats:sup>\u2212\/\u2212<\/jats:sup> mice ( P &lt; 0.05 and P &lt; 0.001, respectively), indicating a protective effect of UCP3 against fat gain. No differences in whole body oxygen consumption were detected following long-term HF feeding. Glucose and insulin tolerance tests revealed that both the UCP3tg and Ucp3<jats:sup>\u2212\/\u2212<\/jats:sup> mice were more glucose tolerant and insulin sensitive compared with wild-type mice after short-term HF feeding, but this protection was not maintained in the long term. Findings indicate that UCP3 is involved in protection from fat gain induced by long-term HF feeding, but not in protection from insulin resistance. <\/jats:p>","DOI":"10.1152\/ajpendo.00779.2007","type":"journal-article","created":{"date-parts":[[2008,8,20]],"date-time":"2008-08-20T04:18:28Z","timestamp":1219205908000},"page":"E1018-E1024","source":"Crossref","is-referenced-by-count":56,"title":["Long-term high-fat feeding induces greater fat storage in mice lacking UCP3"],"prefix":"10.1152","volume":"295","author":[{"given":"Sheila R.","family":"Costford","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shehla N.","family":"Chaudhry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sean 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