{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T10:21:14Z","timestamp":1698574874694},"reference-count":27,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[1980,8,1]]},"abstract":"<jats:p>The involvement of Ca2+ in the impaired insulin release of diabetic C57BL\/KsJ-db\/db mice was studied. Twenty-week-old severely hyperglycemic mice were compared to nondiabetic C57BL\/KsJ mice as controls. Collagenase-isolated islets were maintained for 46 h in tissue culture allowing for equilibration at the same glucose concentration (8.3) mM). The insulin content of both types of islets was similar. In control islets preloaded during culture with 45Ca2+ glucose-induced insulin release was associated with increased 45Ca2+ effux. Islets from diabetic mice showed markedly reduced insulin response to glucose and a smaller increase in 45Ca2+ efflux. Because insulin release was strikingly potentiated by 3-isobutyl-1-methylxanthine (IBMX), even more than in control islets, there was no generalized release defect. In both types of islets, IBMX potentiation was accompanied by a further enhanced 45Ca2+ efflux, possibly suggesting that cAMP effects are associated with increased cytosol Ca2+% concentrations. As Ca2+ uptake was stimulated by glucose in both types of islets, a defect may lie in the mechanism by which glucose uses cellulr calcium to raise cytosol Ca2+ in the beta-cell of these diabetic mice.<\/jats:p>","DOI":"10.1152\/ajpendo.1980.239.2.e132","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T21:16:31Z","timestamp":1513890991000},"page":"E132-E138","source":"Crossref","is-referenced-by-count":2,"title":["Role of Ca2+ in impaired insulin release from islets of diabetic (C57BL\/KsJ-db\/db) mice"],"prefix":"10.1152","volume":"239","author":[{"given":"E. G.","family":"Siegel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. B.","family":"Wollheim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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