{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T01:03:12Z","timestamp":1648515792395},"reference-count":5,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[1997,2,1]]},"abstract":"<jats:p> To determine whether an impairment of intracellular glucose metabolism causes insulin resistance, we examined the effects of suppression of glycolysis or glycogen synthesis on whole body and skeletal muscle insulin-stimulated glucose uptake during 450-min hyperinsulinemic euglycemic clamps in conscious rats. After the initial 150 min to attain steady-state insulin action, animals received an additional infusion of saline, Intralipid and heparin (to suppress glycolysis), or amylin (to suppress glycogen synthesis) for up to 300 min. Insulin-stimulated whole body glucose fluxes were constant with saline infusion (n = 7). In contrast, Intralipid infusion (n = 7) suppressed glycolysis by approximately 32%, and amylin infusion (n = 7) suppressed glycogen synthesis by approximately 45% within 30 min after the start of the infusions (P &lt; 0.05). The suppression of metabolic fluxes increased muscle glucose 6-phosphate levels (P &lt; 0.05), but this did not immediately affect insulin-stimulated glucose uptake due to compensatory increases in other metabolic fluxes. Insulin-stimulated whole body glucose uptake started to decrease at approximately 60 min and was significantly decreased by approximately 30% at the end of clamps (P &lt; 0.05). Similar patterns of changes in insulin-stimulated glucose fluxes were observed in individual skeletal muscles. Thus the suppression of intracellular glucose metabolism caused decreases in insulin-stimulated glucose uptake through a cellular adaptive mechanism in response to a prolonged elevation of glucose 6-phosphate rather than the classic mechanism involving glucose 6-phosphate inhibition of hexokinase. <\/jats:p>","DOI":"10.1152\/ajpendo.1997.272.2.e288","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T10:05:55Z","timestamp":1513937155000},"page":"E288-E296","source":"Crossref","is-referenced-by-count":7,"title":["Prolonged suppression of glucose metabolism causes insulin resistance in rat skeletal muscle"],"prefix":"10.1152","volume":"272","author":[{"given":"J. K.","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, University of SouthernCalifornia School of Medicine, Los Angeles 90033, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. H.","family":"Youn","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of SouthernCalifornia School of Medicine, Los Angeles 90033, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"p_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118349"},{"key":"p_4","doi-asserted-by":"publisher","DOI":"10.1016\/0020-711X(90)90030-7"},{"key":"p_5","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117252"},{"key":"p_7","doi-asserted-by":"publisher","DOI":"10.1007\/BF00253736"},{"key":"p_14","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113758"}],"container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpendo.1997.272.2.E288","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T17:50:32Z","timestamp":1567965032000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpendo.1997.272.2.E288"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,2,1]]},"references-count":5,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,2,1]]}},"alternative-id":["10.1152\/ajpendo.1997.272.2.E288"],"URL":"https:\/\/doi.org\/10.1152\/ajpendo.1997.272.2.e288","relation":{},"ISSN":["0193-1849","1522-1555"],"issn-type":[{"value":"0193-1849","type":"print"},{"value":"1522-1555","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,2,1]]}}}