{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T21:02:32Z","timestamp":1722718952574},"reference-count":1,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[1997,6,1]]},"abstract":"<jats:p> A dietary copper (Cu) deficiency is associated with a twofold increase in hepatic fatty acid biosynthesis. We hypothesized that the induction of hepatic lipogenesis associated with a dietary Cu deficiency reflected an enhanced expression of genes encoding lipogenic enzymes, i.e., fatty acid synthase (FAS). Male weanling rats were pair-meal fed for 42 days a high-sucrose diet that was Cu deficient (CuD; 0.7 microgram Cu\/g) or Cu adequate (CuA; 5.0 micrograms Cu\/g). The CuD diet increased FAS enzymatic activity twofold (P &lt; 0.05). This rise in enzymatic activity was accompanied by a threefold increase in FAS mRNA and a 2.5-fold increase in FAS gene transcription (P &lt; 0.05). Neither the mRNA abundance nor the rate of gene transcription for phosphoenolpyruvate carboxykinase or beta-actin was affected by the CuD diet. The induction of FAS gene transcription was associated with a 65-85% increase in hepatic reduced glutathione (GSH; P &lt; 0.05). When hepatic GSH synthesis was suppressed by treating CuD rats with L-buthionine sulfoximine, the induction of FAS expression was completely prevented. Similarly, feeding N-acetylcysteine to CuA rats increased hepatic GSH levels 2.5-fold, and this was accompanied by a significant induction in FAS expression. These data indicate that the increase in hepatic lipogenesis associated with a Cu deficiency reflects an induction in hepatic lipogenic gene transcription (i.e., FAS) and that the rate of gene transcription may be dependent on hepatic thiol redox. <\/jats:p>","DOI":"10.1152\/ajpendo.1997.272.6.e1124","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T11:36:49Z","timestamp":1513942609000},"page":"E1124-E1129","source":"Crossref","is-referenced-by-count":0,"title":["Hepatic fatty acid synthase gene transcription is induced by a dietary copper deficiency"],"prefix":"10.1152","volume":"272","author":[{"given":"J.","family":"Wilson","sequence":"first","affiliation":[{"name":"Department of Food Science and Human Nutrition, Colorado StateUniversity, Fort Collins 80523, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Food Science and Human Nutrition, Colorado StateUniversity, Fort Collins 80523, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. G.","family":"Allen","sequence":"additional","affiliation":[{"name":"Department of Food Science and Human Nutrition, Colorado StateUniversity, Fort Collins 80523, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Baillie","sequence":"additional","affiliation":[{"name":"Department of Food Science and Human Nutrition, Colorado StateUniversity, Fort Collins 80523, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. D.","family":"Clarke","sequence":"additional","affiliation":[{"name":"Department of Food Science and Human Nutrition, Colorado StateUniversity, Fort Collins 80523, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"p_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(78)90062-X"}],"container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpendo.1997.272.6.E1124","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T18:02:35Z","timestamp":1567965755000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpendo.1997.272.6.E1124"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,6,1]]},"references-count":1,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1997,6,1]]}},"alternative-id":["10.1152\/ajpendo.1997.272.6.E1124"],"URL":"https:\/\/doi.org\/10.1152\/ajpendo.1997.272.6.e1124","relation":{},"ISSN":["0193-1849","1522-1555"],"issn-type":[{"value":"0193-1849","type":"print"},{"value":"1522-1555","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,6,1]]}}}