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For the experiments described in this report rat myometrial tissue was used, after the tissue was loaded with fura 2, to perform cytosolic calcium imaging studies and to perform computer-digitalized in vitro isometric contraction studies. Consistent with the above hypothesis, the cytosolic calcium-imaging studies demonstrated PGF<jats:sub>2\u03b1<\/jats:sub>-stimulated cytosolic calcium oscillations occurring simultaneously with phasic contractions. The in vitro isometric contraction studies confirmed that previously reported inhibitors of the phosphatidylinositol-signaling pathway and cytosolic calcium oscillation mechanisms resulted in significant inhibition of PGF<jats:sub>2\u03b1<\/jats:sub>-stimulated phasic myometrial contractions. In summary, these studies have provided substantial support for the hypothesis that PGF<jats:sub>2\u03b1<\/jats:sub>-stimulated phasic myometrial contractions are generated by intracellular signaling mechanisms involving activation of the phosphatidylinositol-signaling pathway and the production of cytosolic calcium oscillation-like phenomena.<\/jats:p>","DOI":"10.1152\/ajpendo.1997.273.4.e665","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T10:58:02Z","timestamp":1513940282000},"page":"E665-E673","source":"Crossref","is-referenced-by-count":5,"title":["Intracellular mechanisms underlying prostaglandin F<sub>2\u03b1<\/sub>-stimulated phasic myometrial contractions"],"prefix":"10.1152","volume":"273","author":[{"given":"Mark","family":"Phillippe","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Chicago, Chicago, Illinois 60637"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trevania","family":"Saunders","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Chicago, Chicago, Illinois 60637"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Basa","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Chicago, Chicago, Illinois 60637"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.265.4.C976"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1038\/361315a0"},{"key":"B3","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1016\/S0022-3565(25)22497-1","volume":"270","author":"Criswell K. 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