{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,13]],"date-time":"2025-04-13T01:18:33Z","timestamp":1744507113600},"reference-count":38,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Endocrinology and Metabolism"],"published-print":{"date-parts":[[2001,7,1]]},"abstract":"<jats:p>Parathyroid cells express Ca<jats:sup>2+<\/jats:sup>-conducting currents that are activated by raising the extracellular Ca<jats:sup>2+<\/jats:sup>concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>o<\/jats:sub>). We investigated the sensitivity of these currents to dihydropyridines, the expression of voltage-dependent Ca<jats:sup>2+<\/jats:sup>channel (VDCC) subunits, and the effects of dihydropyridines on the intracellular free [Ca<jats:sup>2+<\/jats:sup>] ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) and secretion in these cells. Dihydropyridine channel antagonists dose dependently suppressed Ca<jats:sup>2+<\/jats:sup>-conducting currents, and agonists partially reversed the inhibitory effects of the antagonists in these cells. From a bovine parathyroid cDNA library, we isolated cDNA fragments encoding parts of an \u03b1<jats:sub>1S<\/jats:sub>- and a \u03b2<jats:sub>3<\/jats:sub>-subunit of L-type Ca<jats:sup>2+<\/jats:sup>channels. The \u03b1<jats:sub>1S<\/jats:sub>-subunit cDNA from the parathyroid represents an alternatively spliced variant lacking exon 29 of the corresponding gene. Northern blot analysis and immunocytochemistry confirmed the presence of transcripts and proteins for \u03b1<jats:sub>1<\/jats:sub>- and \u03b2<jats:sub>3<\/jats:sub>-subunits in the parathyroid gland. The addition of dihydropyridines had no significant effects on high [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>o<\/jats:sub>-induced changes in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>and parathyroid hormone (PTH) release. Thus our studies indicate that parathyroid cells express alternatively spliced L-type Ca<jats:sup>2+<\/jats:sup>channel subunits, which do not modulate acute intracellular Ca<jats:sup>2+<\/jats:sup>responses or changes in PTH release.<\/jats:p>","DOI":"10.1152\/ajpendo.2001.281.1.e180","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T18:23:06Z","timestamp":1513966986000},"page":"E180-E189","source":"Crossref","is-referenced-by-count":8,"title":["Parathyroid cells express dihydropyridine-sensitive cation currents and L-type calcium channel subunits"],"prefix":"10.1152","volume":"281","author":[{"given":"Wenhan","family":"Chang","sequence":"first","affiliation":[{"name":"Endocrine Research Unit, Department of Medicine, Veterans Affairs Medical Center, University of California, San Francisco, California 04121;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stacy 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