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Electrostimulation at voltages \u2265170 V resulted in cellular contraction with no detrimental effect on cellular integrity. At 200 V and 4 Hz, palmitate uptake (measured after 3-min incubation) was enhanced 1.5-fold. In both quiescent and contracting myocytes, after their uptake, palmitate was largely and rapidly esterified, mainly into triacylglycerols. Palmitate oxidation (measured after 30 min) contributed to 22% of palmitate taken up by quiescent cardiomyocytes and, after stimulation at 4 Hz, was increased 2.8-fold to contribute to 39% of palmitate utilization. The electrostimulation-mediated increase in palmitate uptake was blocked in the presence of either verapamil, a contraction inhibitor, or sulfo- N-succinimidyl-FA esters, specific inhibitors of FAT\/CD36. These data indicate that, in contracting cardiac myocytes, palmitate uptake is increased due to increased flux through FAT\/CD36.<\/jats:p>","DOI":"10.1152\/ajpendo.2001.281.4.e704","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T19:44:53Z","timestamp":1513971893000},"page":"E704-E712","source":"Crossref","is-referenced-by-count":47,"title":["Electrostimulation enhances FAT\/CD36-mediated long-chain fatty acid uptake by isolated rat cardiac myocytes"],"prefix":"10.1152","volume":"281","author":[{"given":"J. J. F. 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