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Microvascular endothelial cells and preadipocytes were isolated from human omental and subcutaneous adipose tissue biopsies by use of a developed procedure of collagenase digest, immunoselection, and differential trypsinization. Conditioned medium from microvascular endothelial cell cultures promoted the proliferation of preadipocytes ( P = &lt;0.001) and (to a lesser extent) other cell types. No depot-specific differences in mitogenic capacity of microvascular endothelial cell medium or of preadipocyte response were observed. These results indicate that adipose tissue endothelial cells secrete soluble adipogenic factor(s).<\/jats:p>","DOI":"10.1152\/ajpendo.2001.281.5.e1037","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T14:57:52Z","timestamp":1513954672000},"page":"E1037-E1044","source":"Crossref","is-referenced-by-count":131,"title":["Human adipose tissue endothelial cells promote preadipocyte proliferation"],"prefix":"10.1152","volume":"281","author":[{"given":"L. J.","family":"Hutley","sequence":"first","affiliation":[{"name":"Department of Diabetes and Endocrinology and University of Queensland Department of Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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