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Eicosapentaenoic acid (EPA), a polyunsaturated fatty acid, exerted a 50-fold upregulation of UCP-2 that was concentration dependent. This effect was seen within 12 h and was maximal by 36 h. Aspirin blocked the induction of UCP-2 by EPA, indicating involvement of the prostaglandin pathway. Hepatocytes treated with arachidonic acid, the immediate precursor to the prostaglandins, also exhibited an aspirin-inhibitable increase in UCP-2 levels, further supporting the involvement of prostaglandins in regulating hepatic UCP-2. The peroxisome proliferator-activated receptor-\u03b1 (PPAR\u03b1) agonist Wy-14643 stimulated UCP-2 mRNA levels as effectively as EPA. These data indicate that UCP-2 is upregulated by polyunsaturated fatty acids, potentially through a prostaglandin\/PPAR\u03b1-mediated pathway.<\/jats:p>","DOI":"10.1152\/ajpendo.2001.281.6.e1197","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T19:29:14Z","timestamp":1513970954000},"page":"E1197-E1204","source":"Crossref","is-referenced-by-count":61,"title":["Polyunsaturated fatty acids stimulate hepatic UCP-2 expression via a PPAR\u03b1-mediated pathway"],"prefix":"10.1152","volume":"281","author":[{"given":"Michael B.","family":"Armstrong","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Howard C.","family":"Towle","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota 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