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Contrary to our expectations, OVX increased body weight (12\u201318%), bone mineral content (11%), bone length (4%), bone size (3%), and serum, liver, and bone IGF-I (30\u201350%) and decreased total body fat (18%) at 3 wk postsurgery. To determine whether estrogen is the key ovarian factor responsible for these changes, we performed a second experiment in which OVX mice were treated with placebo or estrogen implants. In addition to observing similar results compared with our first experiment, estrogen treatment partially rescued the increased body weight and bone size and completely rescued body fat and IGF-I levels. The increased bone accretion in OVX mice was due to increased bone formation rate (as determined by bone histomorphometry) and increased serum procollagen peptide. In conclusion, contrary to the known estrogen effect as an initiator of GH\/IGF surge and thereby pubertal growth spurt, our findings demonstrate that loss of estrogen and\/or other hormones during the prepubertal growth period effect leads to an increase in IGF-I production and bone accretion in mice. <\/jats:p>","DOI":"10.1152\/ajpendo.90507.2008","type":"journal-article","created":{"date-parts":[[2008,9,24]],"date-time":"2008-09-24T01:06:26Z","timestamp":1222218386000},"page":"E1172-E1180","source":"Crossref","is-referenced-by-count":27,"title":["Prepubertal OVX increases IGF-I expression and bone accretion in C57BL\/6J mice"],"prefix":"10.1152","volume":"295","author":[{"given":"Kristen E.","family":"Govoni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon E.","family":"Wergedal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert B.","family":"Chadwick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Apurva K.","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subburaman","family":"Mohan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650080407"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.2001.280.2.E287"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/8756-3282(92)90358-4"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1210\/endo.134.5.8156931"},{"key":"R6","unstructured":"Garn MS. 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