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Female Sprague-Dawley rats fed a low vitamin A diet were pretreated with RA (po), a low dose of lipopolysaccharide (LPS, ip), or their combination. Twelve hours later, albumin-bound [<jats:sup>3<\/jats:sup>H]RA was injected intravenously, and tissue organic- and aqueous-phase<jats:sup>3<\/jats:sup>H was determined after 10 and 30 min. In liver and plasma,<jats:sup>3<\/jats:sup>H-labeled organic metabolites (e.g., 4-oxo- and 4-hydroxy-RA) were isolated by solid-phase extraction. LPS-induced inflammation significantly reduced plasma retinol by 47%, increased total<jats:sup>3<\/jats:sup>H in plasma at 10 min, and reduced total<jats:sup>3<\/jats:sup>H in liver at both times. In contrast, RA pretreatment did not affect plasma retinol, significantly increased total<jats:sup>3<\/jats:sup>H in plasma at both times, and did not affect liver total<jats:sup>3<\/jats:sup>H. However, by 30 min, RA significantly increased [<jats:sup>3<\/jats:sup>H]RA metabolism in plasma, liver, lung, and small intestine, as indicated by greater<jats:sup>3<\/jats:sup>H-labeled aqueous-phase and<jats:sup>3<\/jats:sup>H-labeled organic-phase metabolites. The results presented here demonstrate that, although LPS-induced inflammation affects the organ distribution of RA, the ability of RA to induce its own catabolism is maintained during inflammation. 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