{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:26:37Z","timestamp":1791120397881,"version":"4.1.0"},"reference-count":53,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:p>The epithelial Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup>channels TRPV5 and TRPV6 are localized to the brush border membrane of intestinal cells and constitute the postulated rate-limiting entry step of active Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup>absorption. The aim of the present study was to investigate the hormonal regulation of these channels. To this end, the effect of 17\u03b2-estradiol (17\u03b2-E<jats:sub>2<\/jats:sub>), 1,25-dihydroxyvitamin D<jats:sub>3<\/jats:sub>[1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>], and dietary Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup>on the expression of the duodenal Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup>transport proteins was investigated in vivo and analyzed using realtime quantitative PCR. Supplementation with 17\u03b2-E<jats:sub>2<\/jats:sub>increased duodenal gene expression of TRPV5 and TRPV6 but also calbindin-D<jats:sub>9K<\/jats:sub>and plasma membrane Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup>-ATPase (PMCA1b) in ovariectomized rats. 25-Hydroxyvitamin D<jats:sub>3<\/jats:sub>-1\u03b1-hydroxylase (1\u03b1-OHase) knockout mice are characterized by hyperparathyroidism, rickets, hypocalcemia, and undetectable levels of 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>and were used to study the 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>-dependency of the stimulatory effects of 17\u03b2-E<jats:sub>2<\/jats:sub>. Treatment with 17\u03b2-E<jats:sub>2<\/jats:sub>upregulated mRNA levels of duodenal TRPV6 in these 1\u03b1-OHase knockout mice, which was accompanied by increased serum Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup>concentrations from 1.69 \u00b1 0.10 to 2.03 \u00b1 0.12 mM ( P &lt; 0.05). In addition, high dietary Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup>intake normalized serum Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup>in these mice and upregulated expression of genes encoding the duodenal Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup>transport proteins except for PMCA1b. Supplementation with 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>resulted in increased expression of TRPV6, calbindin-D<jats:sub>9K<\/jats:sub>, and PMCA1b and normalization of serum Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup>. Expression levels of duodenal TRPV5 mRNA are below detection limits in these 1\u03b1-OHase knockout mice, but supplementation with 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>upregulated the expression to significant levels. In conclusion, TRPV5 and TRPV6 are regulated by 17\u03b2-E<jats:sub>2<\/jats:sub>and 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>, whereas dietary Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup>is positively involved in the regulation of TRPV6 only.<\/jats:p>","DOI":"10.1152\/ajpgi.00036.2003","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T19:58:13Z","timestamp":1425412693000},"page":"G78-G85","source":"Crossref","is-referenced-by-count":147,"title":["Regulation of the epithelial Ca<sup>2<\/sup><sup>+<\/sup>channels in small intestine as studied by quantitative mRNA detection"],"prefix":"10.1152","volume":"285","author":[{"given":"Monique","family":"van Abel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joost G. J.","family":"Hoenderop","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annemiete W. C. M.","family":"van der Kemp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes P. T. M.","family":"van Leeuwen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9 J. 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