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Mucosal addition of LLO, but not a LLO mutant, induced a dose- and pH-dependent increase in short-circuit current ( I<jats:sub>SC<\/jats:sub>). Sodium and chloride tracer flux and DIDS sensitivity studies revealed that I<jats:sub>SC<\/jats:sub>was mainly due to electrogenic chloride secretion. Barrier function was impaired by LLO, as assessed by transepithelial resistance ( R<jats:sup>t<\/jats:sup>) and mannitol flux measurements. Intracellular signal transduction occurred through Ca<jats:sup>2+<\/jats:sup>release from intracellular stores and PKC activation. In conclusion, listeriolysin induces chloride secretion and perturbs epithelial barrier function, thus potentially contributing to Listeria-induced diarrhea.<\/jats:p>","DOI":"10.1152\/ajpgi.00040.2009","type":"journal-article","created":{"date-parts":[[2009,4,17]],"date-time":"2009-04-17T00:54:45Z","timestamp":1239929685000},"page":"G1350-G1359","source":"Crossref","is-referenced-by-count":21,"title":["Listeriolysin O affects barrier function and induces chloride secretion in HT-29\/B6 colon epithelial cells"],"prefix":"10.1152","volume":"296","author":[{"given":"Jan F.","family":"Richter","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfred H.","family":"Gitter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothee","family":"G\u00fcnzel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siegfried","family":"Weiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walid","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trinad","family":"Chakraborty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Fromm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg D.","family":"Schulzke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3592-3601.2002"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200004273421702"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0450.2000.00371.x"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.62.1.535"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)90411-W"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1038\/27094"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2006.09.002"},{"key":"R8","unstructured":"Bittenbrink JT.Elektrophysiologische Charakterisierung und Pharmakologische Blockade von Listeriolysin-O Induzierten Membranporen(PhD thesis). 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