{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T04:31:57Z","timestamp":1747801917715,"version":"3.41.0"},"reference-count":29,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2002,4,1]]},"abstract":"<jats:p>We investigated Cl<jats:sup>\u2212<\/jats:sup>transport pathways in the apical and basolateral membranes of rabbit esophageal epithelial cells (EEC) using conventional and ion-selective microelectrodes. Intact sections of esophageal epithelium were mounted serosal or luminal side up in a modified Ussing chamber, where transepithelial potential difference and transepithelial resistance could be determined. Microelectrodes were used to measure intracellular Cl<jats:sup>\u2212<\/jats:sup>activity (a[Formula: see text]), basolateral or apical membrane potentials ( V<jats:sub>mBL<\/jats:sub>or V<jats:sub>mC<\/jats:sub>), and the voltage divider ratio. When a basal cell was impaled, V<jats:sub>mBL<\/jats:sub>was \u221273 \u00b1 4.3 mV and a[Formula: see text] was 16.4 \u00b1 2.1 mM, which were similar in presence or absence of bicarbonate. Removal of serosal Cl<jats:sup>\u2212<\/jats:sup>caused a transient depolarization of V<jats:sub>mBL<\/jats:sub>and a decrease in a[Formula: see text] of 6.5 \u00b1 0.9 mM. The depolarization and the rate of decrease of a[Formula: see text] were inhibited by \u223c60% in the presence of the Cl<jats:sup>\u2212<\/jats:sup>-channel blocker flufenamate. Serosal bumetanide significantly decreased the rate of change of a[Formula: see text] on removal and readdition of serosal Cl<jats:sup>\u2212<\/jats:sup>. When a luminal cell was impaled, V<jats:sub>mC<\/jats:sub>was \u221265 \u00b1 3.6 mV and a[Formula: see text] was 16.3 \u00b1 2.2 mM. Removal of luminal Cl<jats:sup>\u2212<\/jats:sup>depolarized V<jats:sub>mC<\/jats:sub>and decreased a[Formula: see text] by only 2.5 \u00b1 0.9 mM. Subsequent removal of Cl<jats:sup>\u2212<\/jats:sup>from the serosal bath decreased a[Formula: see text]in the luminal cell by an additional 6.4 \u00b1 1.0 mM. A plot of V<jats:sub>mBL<\/jats:sub>measurements vs. log a[Formula: see text]\/log a[Formula: see text] (a[Formula: see text] is the activity of Cl<jats:sup>\u2212<\/jats:sup>in a luminal or serosal bath) yielded a straight line [slope ( S) = 67.8 mV\/decade of change in a[Formula: see text]\/a[Formula: see text]]. In contrast, V<jats:sub>mC<\/jats:sub>correlated very poorly with log a[Formula: see text]\/a[Formula: see text] ( S = 18.9 mV\/decade of change in a[Formula: see text]\/a[Formula: see text]). These results indicate that 1) in rabbit EEC, a[Formula: see text] is higher than equilibrium across apical and basolateral membranes, and this process is independent of bicarbonate; 2) the basolateral cell membrane possesses a conductive Cl<jats:sup>\u2212<\/jats:sup>pathway sensitive to flufenamate; and 3) the apical membrane has limited permeability to Cl<jats:sup>\u2212<\/jats:sup>, which is consistent with the limited capacity for transepithelial Cl<jats:sup>\u2212<\/jats:sup>transport. Transport of Cl<jats:sup>\u2212<\/jats:sup>at the basolateral membrane is likely the dominant pathway for regulation of intracellular Cl<jats:sup>\u2212<\/jats:sup>.<\/jats:p>","DOI":"10.1152\/ajpgi.00085.2001","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T19:55:14Z","timestamp":1425412514000},"page":"G663-G675","source":"Crossref","is-referenced-by-count":5,"title":["Chloride transport in rabbit esophageal epithelial cells"],"prefix":"10.1152","volume":"282","author":[{"given":"Solange","family":"Abdulnour-Nakhoul","sequence":"first","affiliation":[{"name":"Departments of Medicine and Physiology, Tulane University School of Medicine, and Veterans Administration Medical Center, New Orleans, Louisiana 70112-2699"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nazih L.","family":"Nakhoul","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Physiology, Tulane University School of Medicine, and Veterans 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