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For example, EPEC-induced ATP release was potentiated by noncytotoxic agents that elevate host cell cAMP, such as forskolin and cholera toxin, and by exposure to hypotonic medium. These findings and the performance of the EPEC espF mutant led us to hypothesize that the CFTR plays a role in EPEC-induced ATP release that is independent of cell death. We report the results of experiments using specific, cell-permeable CFTR activators and inhibitors, as well as transfection of the CFTR into non-CFTR-expressing cell lines, which incriminate the CFTR as a second pathway for ATP release from host cells. Increased ATP release via CFTR is not accompanied by an increase in EPEC adherence to transfected cells. The CFTR-dependent ATP release pathway becomes activated endogenously later in EPEC infection, and this activation is mediated, at least in part, by generation of extracellular adenosine from the breakdown of released ATP.<\/jats:p>","DOI":"10.1152\/ajpgi.00137.2005","type":"journal-article","created":{"date-parts":[[2005,8,10]],"date-time":"2005-08-10T18:28:15Z","timestamp":1123698495000},"page":"G407-G417","source":"Crossref","is-referenced-by-count":25,"title":["Two pathways for ATP release from host cells in enteropathogenic<i>Escherichia coli<\/i>infection"],"prefix":"10.1152","volume":"289","author":[{"given":"John K.","family":"Crane","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tonniele M.","family":"Naeher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shilpa 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