{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T02:26:20Z","timestamp":1791080780490,"version":"4.1.0"},"reference-count":48,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:p>Alterations in the intestinal microbiota have been suggested as an etiological factor in the pathogenesis of irritable bowel syndrome (IBS). This study used a molecular fingerprinting technique to compare the composition and biodiversity of the microbiota within fecal and mucosal niches between patients with diarrhea-predominant IBS (D-IBS) and healthy controls. Terminal-restriction fragment (T-RF) length polymorphism (T-RFLP) fingerprinting of the bacterial 16S rRNA gene was used to perform microbial community composition analyses on fecal and mucosal samples from patients with D-IBS ( n = 16) and healthy controls ( n = 21). Molecular fingerprinting of the microbiota from fecal and colonic mucosal samples revealed differences in the contribution of T-RFs to the microbiota between D-IBS patients and healthy controls. Further analysis revealed a significantly lower (1.2-fold) biodiversity of microbes within fecal samples from D-IBS patients than healthy controls ( P = 0.008). No difference in biodiversity in mucosal samples was detected between D-IBS patients and healthy controls. Multivariate analysis of T-RFLP profiles demonstrated distinct microbial communities between luminal and mucosal niches in all samples. Our findings of compositional differences in the luminal- and mucosal-associated microbiota between D-IBS patients and healthy controls and diminished microbial biodiversity in D-IBS fecal samples further support the hypothesis that alterations in the intestinal microbiota may have an etiological role in the pathogenesis of D-IBS and suggest that luminal and mucosal niches need to be investigated.<\/jats:p>","DOI":"10.1152\/ajpgi.00154.2011","type":"journal-article","created":{"date-parts":[[2011,7,8]],"date-time":"2011-07-08T11:16:10Z","timestamp":1310123770000},"page":"G799-G807","source":"Crossref","is-referenced-by-count":251,"title":["Molecular analysis of the luminal- and mucosal-associated intestinal microbiota in diarrhea-predominant irritable bowel syndrome"],"prefix":"10.1152","volume":"301","author":[{"given":"Ian M.","family":"Carroll","sequence":"first","affiliation":[{"name":"Division of Gastroenterology and Hepatology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamar","family":"Ringel-Kulka","sequence":"additional","affiliation":[{"name":"Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Temitope O.","family":"Keku","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young-Hyo","family":"Chang","sequence":"additional","affiliation":[{"name":"Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher D.","family":"Packey","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Center for Gastrointestinal Biology and Disease,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Balfour","family":"Sartor","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yehuda","family":"Ringel","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002836"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2006.00875.x"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1126\/science.1104816"},{"key":"B4","first-page":"185","volume":"5","author":"Balsari A","year":"1982","journal-title":"Microbiologica"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1007\/BF01537265"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1186\/1757-4749-2-19"},{"key":"B7","first-page":"55","volume-title":"Probiotics: A Clinical Guide","author":"Carroll IM","year":"2009"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1097\/MCG.0b013e318204593e"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-009-0934-x"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2008.37"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-010-9738-y"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2010.08.004"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2011.04633.x"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.280.3.G368"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.04.005"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1186\/1471-230X-9-95"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.2165\/00019053-200119060-00003"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2005.031708"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1126\/science.1155725"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.11.061"},{"key":"B21","first-page":"37","volume":"62","author":"Lydiard RB","year":"2001","journal-title":"J Clin Psychiatry"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.15.5936"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2005.40312.x"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2005.073817"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsim.2004.08.009"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46134-0"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2008.167270"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2003.025403"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2004.tb03544.x"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2005.00745.x"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1017\/S002966511000011X"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1004409"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-145-8-200610170-00004"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1016\/j.giec.2008.12.004"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1097\/MCG.0b013e31820ca4d6"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00312-06"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1136\/gut.52.2.237"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2006.00458.x"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.4161\/gmic.1.3.12360"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-006-9106-0"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v10.i12.1802"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.3109\/00365521.2011.565066"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1007\/s11894-009-0039-x"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.7.3380-3389.2005"},{"key":"B46","first-page":"512","volume":"22","author":"Tana C","year":"2009","journal-title":"Neurogastroenterol Motil"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.08.049"},{"key":"B48","doi-asserted-by":"crossref","first-page":"3854","DOI":"10.1128\/AEM.64.10.3854-3859.1998","volume":"64","author":"Zoetendal EG","year":"1998","journal-title":"Appl Environ Microbiol"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.7.3401-3407.2002"}],"container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.00154.2011","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,6,21]],"date-time":"2020-06-21T02:03:37Z","timestamp":1592705017000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.00154.2011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11]]},"references-count":48,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2011,11]]}},"alternative-id":["10.1152\/ajpgi.00154.2011"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.00154.2011","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,11]]}}}