{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T01:38:03Z","timestamp":1792460283168,"version":"4.5.0"},"reference-count":60,"publisher":"American Physiological Society","issue":"8","funder":[{"name":"NIH","award":["R01 DK080047"],"award-info":[{"award-number":["R01 DK080047"]}]},{"name":"NIH","award":["R01 DK074192"],"award-info":[{"award-number":["R01 DK074192"]}]},{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"crossref","award":["524-2014-1"],"award-info":[{"award-number":["524-2014-1"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2015,10,15]]},"abstract":"<jats:p>\n                    Divalent metal-ion transporter-1 (DMT1) is a widely expressed iron-preferring membrane-transport protein that serves a critical role in erythroid iron utilization. We have investigated its role in intestinal metal absorption by studying a mouse model lacking intestinal DMT1 (i.e., DMT1\n                    <jats:sup>int\/int<\/jats:sup>\n                    ). DMT1\n                    <jats:sup>int\/int<\/jats:sup>\n                    mice exhibited a profound hypochromic-microcytic anemia, splenomegaly, and cardiomegaly. That the anemia was due to iron deficiency was demonstrated by the following observations in DMT1\n                    <jats:sup>int\/int<\/jats:sup>\n                    mice: 1) blood iron and tissue nonheme-iron stores were depleted; 2) mRNA expression of liver hepcidin (Hamp1) was depressed; and 3) intraperitoneal iron injection corrected the anemia, and reversed the changes in blood iron, nonheme-iron stores, and hepcidin expression levels. We observed decreased total iron content in multiple tissues from DMT1\n                    <jats:sup>int\/int<\/jats:sup>\n                    mice compared with DMT1\n                    <jats:sup>+\/+<\/jats:sup>\n                    mice but no meaningful change in copper, manganese, or zinc. DMT1\n                    <jats:sup>int\/int<\/jats:sup>\n                    mice absorbed\n                    <jats:sup>64<\/jats:sup>\n                    Cu and\n                    <jats:sup>54<\/jats:sup>\n                    Mn from an intragastric dose to the same extent as did DMT1\n                    <jats:sup>+\/+<\/jats:sup>\n                    mice but the absorption of\n                    <jats:sup>59<\/jats:sup>\n                    Fe was virtually abolished in DMT1\n                    <jats:sup>int\/int<\/jats:sup>\n                    mice. This study reveals a critical function for DMT1 in intestinal nonheme-iron absorption for normal growth and development. Further, this work demonstrates that intestinal DMT1 is not required for the intestinal transport of copper, manganese, or zinc.\n                  <\/jats:p>","DOI":"10.1152\/ajpgi.00160.2015","type":"journal-article","created":{"date-parts":[[2015,8,20]],"date-time":"2015-08-20T20:21:51Z","timestamp":1440102111000},"page":"G635-G647","source":"Crossref","is-referenced-by-count":148,"title":["Intestinal DMT1 is critical for iron absorption in the mouse but is not required for the absorption of copper or manganese"],"prefix":"10.1152","volume":"309","author":[{"given":"Ali","family":"Shawki","sequence":"first","affiliation":[{"name":"Department of Molecular &amp; Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio;"},{"name":"Systems Biology &amp; Physiology Program, University of Cincinnati College of Medicine, Cincinnati, Ohio;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah R.","family":"Anthony","sequence":"additional","affiliation":[{"name":"Department of Molecular &amp; Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiro","family":"Nose","sequence":"additional","affiliation":[{"name":"Department of Pharmacology &amp; Cancer Biology, Duke University Medical Center, Durham, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melinda A.","family":"Engevik","sequence":"additional","affiliation":[{"name":"Department of Molecular &amp; Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio;"},{"name":"Systems Biology &amp; Physiology Program, University of Cincinnati College of Medicine, Cincinnati, Ohio;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Niespodzany","sequence":"additional","affiliation":[{"name":"Department of Molecular &amp; Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasa","family":"Barrientos","sequence":"additional","affiliation":[{"name":"Department of Pharmacology &amp; Cancer Biology, Duke University Medical Center, Durham, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"\u00d6hrvik","sequence":"additional","affiliation":[{"name":"Department of Pharmacology &amp; Cancer Biology, Duke University Medical Center, Durham, North Carolina;"},{"name":"Department of Medical Biochemistry &amp; Microbiology, Uppsala University, Uppsala, Sweden; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger T.","family":"Worrell","sequence":"additional","affiliation":[{"name":"Department of Molecular &amp; Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio;"},{"name":"Systems Biology &amp; Physiology Program, University of Cincinnati College of Medicine, Cincinnati, Ohio;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis J.","family":"Thiele","sequence":"additional","affiliation":[{"name":"Department of Pharmacology &amp; Cancer Biology, Duke University Medical Center, Durham, North Carolina;"},{"name":"Department of Biochemistry, Duke University Medical Center, Durham, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9661-8838","authenticated-orcid":false,"given":"Bryan","family":"Mackenzie","sequence":"additional","affiliation":[{"name":"Department of Molecular &amp; Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio;"},{"name":"Systems Biology &amp; Physiology Program, University of Cincinnati College of Medicine, Cincinnati, Ohio;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-013-9691-6"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00480.2002"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/138.12.2534"},{"key":"B4","volume-title":"Williams Hematology","author":"Beutler E","year":"2010"},{"key":"B5","doi-asserted-by":"crossref","first-page":"4406","DOI":"10.1182\/blood.V93.12.4406","volume":"93","author":"Canonne-Hergaux F","year":"1999","journal-title":"Blood"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1080\/01926230600867743"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1007\/s003600050085"},{"key":"B8","first-page":"25","volume":"39","author":"Collins JF","year":"2006","journal-title":"Biol Res"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00489.2004"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1024\/0300-9831\/a000030"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2000.279.1.R1"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1017\/S1462399410001687"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddm088"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-141-36720"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1002\/gene.20042"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-011-9243-2"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.3.1148"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1038\/ng0897-383"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1004967"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.50"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00008.2013"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24356"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1038\/41343"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-02-0716"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00021.2014"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192423399"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.364208"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2012.05.011"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.3945\/jn.113.181867"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1021\/bi048768+"},{"key":"B31","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1301\/nr.2007.jul.335\u2013340","volume":"65","author":"Knutson MD","year":"2007","journal-title":"Nutr Rev"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104728200"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208002200"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1039\/C5MT00097A"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0606424103"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00363.2005"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-005-1494-3"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1126\/science.1057206"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80425-6"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1038\/ng.320"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1126\/science.1104742"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2006.08.009"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.143826"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1038\/ng1658"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-02-003681"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00545.2010"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00479.2010"},{"key":"B49","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1096\/fj.14-262733","volume":"29","author":"Prakash S","year":"2015","journal-title":"FASEB J"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1301\/00296640260130731"},{"key":"B51","first-page":"420","volume":"107","author":"Said HM","year":"1986","journal-title":"J Lab Clin Med"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394316-3.00005-3"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E02-03-0165"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/136.12.3010"},{"key":"B55","volume-title":"Iron","author":"Torrance JD","year":"1980"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26401"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.367284"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1086\/341125"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2008.05.010"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.24033"}],"container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.00160.2015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,7]],"date-time":"2020-09-07T07:18:46Z","timestamp":1599463126000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.00160.2015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10,15]]},"references-count":60,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2015,10,15]]}},"alternative-id":["10.1152\/ajpgi.00160.2015"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.00160.2015","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10,15]]}}}