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Administration of 2.5, 5, and 10 mg\/kg ip N<jats:sup>6<\/jats:sup>-iminoethyl-l-lysine [inducible NOS (iNOS) inhibitor] had no effect in either experimental group. Indomethacin (5 mg\/kg sc), NS-398 (cyclooxygenase-2 inhibitor; 10 mg\/kg ip), and dexamethasone (10 mg\/kg sc) but not quinacrine (20 mg\/kg ip) significantly prevented delay in gastric emptying induced by endotoxin but failed to modify gastric emptying in vehicle-treated animals. Ca<jats:sup>2+<\/jats:sup>-dependent NOS activity in the antrum pylorus of the stomach was diminished by endotoxin, whereas Ca<jats:sup>2+<\/jats:sup>-independent NOS activity was not changed. In addition, decreased nNOS mRNA and protein were observed in the antrum pylorus of endotoxin-treated rats. 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