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Absorption of butyrate into the colonocytes is essential for these intracellular effects. Monocarboxylate transporter 1 (MCT1) plays a major role in colonic luminal butyrate absorption. Previous studies (Tan J, McKenzie C, Potamitis M, Thorburn AN, Mackay CR, Macia L. Adv Immunol 121: 91\u2013119, 2014.) showed decreased MCT1 expression and function in intestinal inflammation. We have previously shown (Borthakur A, Gill RK, Hodges K, Ramaswamy K, Hecht G, Dudeja PK. Am J Physiol Gastrointest Liver Physiol 290: G30\u2013G35, 2006.) impaired butyrate absorption in human intestinal epithelial Caco-2 cells due to decreased MCT1 level at the apical cell surface following enteropathogenic E. coli (EPEC) infection. Current studies, therefore, examined the potential role of probiotic Lactobacilli in stimulating MCT1-mediated butyrate uptake and counteracting EPEC inhibition of MCT1 function. Of the five species of Lactobacilli, short-term (3 h) treatment with L. acidophilus (LA) significantly increased MCT1-mediated butyrate uptake in Caco-2 cells. Heat-killed LA was ineffective, whereas the conditioned culture supernatant of LA (LA-CS) was equally effective in stimulating MCT1 function, indicating that the effects are mediated by LA-secreted soluble factor(s). Furthermore, LA-CS increased apical membrane levels of MCT1 protein via decreasing its basal endocytosis, suggesting that LA-CS stimulation of butyrate uptake could be secondary to increased levels of MCT1 on the apical cell surface. LA-CS also attenuated EPEC inhibition of butyrate uptake and EPEC-mediated endocytosis of MCT1. Our studies highlight distinct role of specific LA-secreted molecules in modulating colonic butyrate absorption. <\/jats:p>","DOI":"10.1152\/ajpgi.00186.2015","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T23:09:20Z","timestamp":1439507360000},"page":"G602-G607","source":"Crossref","is-referenced-by-count":68,"title":["<i>Lactobacillus acidophilus<\/i> counteracts enteropathogenic <i>E. coli<\/i>-induced inhibition of butyrate uptake in intestinal epithelial cells"],"prefix":"10.1152","volume":"309","author":[{"given":"Anoop","family":"Kumar","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Gastroenterology &amp; Hepatology, University of Illinois at Chicago, Chicago, Illinois; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waddah A.","family":"Alrefai","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Gastroenterology &amp; Hepatology, University of Illinois at 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