{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T21:59:44Z","timestamp":1788991184493,"version":"build-2803163510"},"reference-count":66,"publisher":"American Physiological Society","issue":"4","funder":[{"name":"Eurolysine"}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2026,4,1]]},"abstract":"<jats:p>The microbiota degrades branched-chain amino acids to produce branched-chain fatty acids, the effects of which on the intestinal epithelial barrier remain poorly described. In this study, we demonstrate that the leucine-derived metabolite isovalerate enhances epithelial barrier function in cell monolayers derived from porcine ileum organoids. We also identified gut microbiota species potentially able to produce branched-chain fatty acids that could be targeted to promote gut health.<\/jats:p>","DOI":"10.1152\/ajpgi.00193.2025","type":"journal-article","created":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T22:20:41Z","timestamp":1772058041000},"page":"G459-G477","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["The gut microbiota metabolite isovalerate enhances the epithelial barrier function in cell monolayers derived from porcine ileum organoids"],"prefix":"10.1152","volume":"330","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1559-2067","authenticated-orcid":false,"given":"Martin","family":"Beaumont","sequence":"first","affiliation":[{"name":"GenPhySE, Universit\u00e9 de Toulouse, INRAE, ENVT","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8205-6486","authenticated-orcid":false,"given":"Cl\u00e1udia M.","family":"Vicente","sequence":"additional","affiliation":[{"name":"GenPhySE, Universit\u00e9 de Toulouse, INRAE, ENVT","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5465-8084","authenticated-orcid":false,"given":"Cristina","family":"Plata-Calzado","sequence":"additional","affiliation":[{"name":"GenPhySE, Universit\u00e9 de Toulouse, INRAE, ENVT","place":["France"]},{"name":"Universidad de Sevilla","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne","family":"Lencina","sequence":"additional","affiliation":[{"name":"GenPhySE, Universit\u00e9 de Toulouse, INRAE, ENVT","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Jones","sequence":"additional","affiliation":[{"name":"GenPhySE, Universit\u00e9 de Toulouse, INRAE, ENVT","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Lecuelle","sequence":"additional","affiliation":[{"name":"Eurolysine","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tristan","family":"Chalvon-Demersay","sequence":"additional","affiliation":[{"name":"METEX Animal Nutrition","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3608"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03852-1"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00042.2024"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2021.02.007"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-019-0704-8"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2021.02.001"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.3945\/jn.109.104638"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0230231"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2024.2430424"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2016.08.011"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2022.1066898"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1128\/aem.35.5.872-877.1978"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10040762"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00319.2017"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.3945\/jn.113.175828"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2022.2108280"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-022-00807-y"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.3390\/nu12010197"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.09.001"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05470-4"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.05.004"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2020.101798"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2016.09.003"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.0c00745"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.983031"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.3791\/64917"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2025.09.009"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2020.1747335"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz248"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v067.i01"},{"key":"B31","unstructured":"Fox J, Weisberg S, Price B, Adler D, Bates D, Baud-Bovy G, Bolker B, Ellison S, Firth D, Friendly M, Gorjanc G, Graves S, Heiberger R, Krivitsky P, Laboissiere R, Maechler M, Monette G, Murdoch D, Nilsson H, Ogle D, Ripley B, Short T, Venables W, Walker S, Winsemius D, Zeileis AR-C. car: Companion to Applied Regression [Online]. https:\/\/cran.r-project.org\/web\/packages\/car\/index.html [6 Jul. 2024]."},{"key":"B32","unstructured":"Lenth RV, Bolker B, Buerkner P, Gin\u00e9-V\u00e1zquez I, Herve M, Jung M, Love J, Miguez F, Piaskowski J, Riebl H, Singmann H. emmeans: Estimated Marginal Means, aka Least-Squares Means [Online]. https:\/\/cran.r-project.org\/web\/packages\/emmeans\/index.html [6 Jul. 2024]."},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-421"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1093\/bioadv\/vbab016"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav1282"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28412-0"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btab007"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(96)00904-5"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1128\/br.41.2.391-418.1977"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1993.tb17858.x"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.12.3504-3511.1995"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.3.780-787.1996"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00889-14"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0179586"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00694-23"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1136\/gut.28.10.1221"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1992.tb04882.x"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.117.158816"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00973"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1700105"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.05.034"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23782-w"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.03278-24"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1007\/BF00402304"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-022-00649-y"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1111\/acel.14434"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-025-04205-y"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2024.2412669"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.110.002188"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.3945\/jn.111.156190"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2015.04.004"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2016.01.028"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.05.018"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114520001385"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1442077"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-025-01239-0"}],"container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpgi.00193.2025","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T19:28:47Z","timestamp":1776972527000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpgi.00193.2025"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,1]]},"references-count":66,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4,1]]}},"alternative-id":["10.1152\/ajpgi.00193.2025"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.00193.2025","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.06.04.657803","asserted-by":"object"}]},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,1]]},"assertion":[{"value":"2025-06-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-12","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}