{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:10:51Z","timestamp":1790485851623,"version":"4.1.0"},"reference-count":35,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2002,2,1]]},"abstract":"<jats:p>The aquaporin (AQP) family of water channels is distributed ubiquitously in many epithelia and plays a fundamental role in transmembrane water transport. The aim of this study is to identify the water transport pathway in pancreatic duct cells where most of the HCO[Formula: see text]-rich fluid originates. Using digital videomicroscopy, we measured the osmotic water permeability ( P<jats:sub>f<\/jats:sub>) of pancreatic duct epithelium by exposing isolated rat interlobular ducts to the hypotonic solution (145 mosM). To identify mRNA and protein of AQPs expressed in duct cells, we conducted RT-PCR analysis and immunohistochemistry of the isolated duct and pancreas. The calculated P<jats:sub>f<\/jats:sub>(160\u2013230 \u03bcm\/s) of the isolated ducts was significantly reduced to 16\u201335 \u03bcm\/s by 80\u201390% with either basolateral or luminal applications of HgCl<jats:sub>2<\/jats:sub>. Fluid secretion evoked by secretin was almost completely abolished by a basolateral or luminal application of HgCl<jats:sub>2<\/jats:sub>. A large amount of AQP1 and small amounts of AQP5 transcripts were detected in the isolated duct cells by RT-PCR. AQP1, but not AQP5, immunoreactivity was present in both luminal and basolateral membranes of the interlobular duct cells. Mercury-sensitive water channels are present in both luminal and basolateral membranes of rat pancreatic ducts. AQP1 of the known AQPs appears to be the main water pathway in interlobular ducts.<\/jats:p>","DOI":"10.1152\/ajpgi.00198.2001","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T19:55:03Z","timestamp":1425412503000},"page":"G324-G331","source":"Crossref","is-referenced-by-count":35,"title":["Aquaporins in rat pancreatic interlobular ducts"],"prefix":"10.1152","volume":"282","author":[{"given":"Shigeru B. 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