{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T06:45:57Z","timestamp":1764053157504},"reference-count":44,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:p>Sympathetic stimulation induces weak salivation compared with parasympathetic stimulation. To clarify this phenomenon in salivary glands, we investigated cAMP-induced modulation of Ca<jats:sup>2+<\/jats:sup>-activated Cl<jats:sup>\u2212<\/jats:sup>secretion from rat parotid and submandibular acinar cells because fluid secretion from salivary glands depends on the Cl<jats:sup>\u2212<\/jats:sup>secretion. Carbachol (Cch), a Ca<jats:sup>2+<\/jats:sup>-increasing agent, induced hyperpolarization of the cells with oscillatory depolarization in the current clamp mode of the gramicidin-perforated patch recording. In the voltage clamp mode at \u221280 mV, Cch induced a bumetanide-sensitive oscillatory inward current, which was larger in rat submandibular acinar cells than in parotid acinar cells. Forskolin and IBMX, cAMP-increasing agents, did not induce any marked current, but they evoked a small nonoscillatory inward current in the presence of Cch and suppressed the Cch-induced oscillatory inward current in all parotid acinar cells and half (56%) of submandibular acinar cells. In the current clamp mode, forskolin + IBMX evoked a small nonoscillatory depolarization in the presence of Cch and reduced the amplitude of Cch-induced oscillatory depolarization in both acinar cells. The oscillatory inward current estimated at the depolarized membrane potential was suppressed by forskolin + IBMX. These results indicate that cAMP suppresses Ca<jats:sup>2+<\/jats:sup>-activated oscillatory Cl<jats:sup>\u2212<\/jats:sup>secretion of parotid and submandibular acinar cells at \u221280 mV and possibly at the membrane potential during Cch stimulation. The suppression may result in the weak salivation induced by sympathetic stimulation.<\/jats:p>","DOI":"10.1152\/ajpgi.00239.2007","type":"journal-article","created":{"date-parts":[[2008,1,11]],"date-time":"2008-01-11T01:34:07Z","timestamp":1200015247000},"page":"G738-G747","source":"Crossref","is-referenced-by-count":9,"title":["Suppression of carbachol-induced oscillatory Cl<sup>\u2212<\/sup>secretion by forskolin in rat parotid and submandibular acinar cells"],"prefix":"10.1152","volume":"294","author":[{"given":"Takahide","family":"Shintani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chikara","family":"Hirono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Sugita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiko","family":"Iwasa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiki","family":"Shiba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1994.72.4.1530"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6107(96)00013-2"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020695"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.108.1.35"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1996.sp021149"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1177\/00220345890680110401"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M406201200"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106609200"},{"key":"R9","unstructured":"Cook DI, Van Lennep EW, Roberts ML, Young JA.Secretion by the major salivary glands. 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