{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:04:39Z","timestamp":1792065879492,"version":"4.4.0"},"reference-count":42,"publisher":"American Physiological Society","issue":"4","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China (NSFC)","doi-asserted-by":"publisher","award":["81760748"],"award-info":[{"award-number":["81760748"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"nature science foundation of Inner mongolia autonomous region","award":["2017MS0831"],"award-info":[{"award-number":["2017MS0831"]}]},{"name":"the major science foundation of affiliated hospital of Inner Mongolia medical university"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2019,4,1]]},"abstract":"<jats:p>\n                    Hepatic fibrosis is chronic liver damage with many causes that has a relatively high death rate. The current study showed that long noncoding RNA (lncRNA) growth arrest-specific transcript 5 (GAS5), microRNA-23a (miR-23a), and phosphatase and tensin homolog (PTEN) play important roles in the pathological process of hepatic fibrosis but have a relatively unclear regulatory mechanism. This study aimed to investigate the roles of lncRNA GAS5, miR-23a, and PTEN in the pathological process of hepatic fibrosis and hepatic stellate cell (HSC) activation. We used carbon tetrachloride (CCl\n                    <jats:sub>4<\/jats:sub>\n                    ) intraperitoneal injections to establish a rat hepatic fibrosis model and exogenous transforming growth factor-\u03b21 to establish an HSC activation model. Quantitative RT-PCR, Western blot, dual-luciferase reporter system, and RNA pull-down assays were used to investigate which microRNAs and lncRNAs participate in the process of hepatic fibrosis and HSC activation. miR-23a expression increased significantly in hepatic fibrosis tissues and activated HSCs. miR-23a interaction with and degradation of PTEN further influenced the downstream signaling pathway phosphatidylinositol 3-kinase\/protein kinase B\/mammalian target of rapamycin\/Snail (PI3K\/Akt\/mTOR\/Snail), causing E-cadherin expression levels to decrease and \u03b1-smooth muscle actin and collagen I expression levels to increase. lncRNA GAS5 can be used as a sponge platform for miR-23a to decrease miR-23a expression levels competitively. We revealed the role of the lncRNA GAS5\/miR-23a\/PTEN\/PI3K\/Akt\/mTOR\/Snail signaling pathway in hepatic fibrosis, providing molecular targets for the treatment of hepatic fibrosis.\n                  <\/jats:p>\n                  <jats:p>NEW &amp; NOTEWORTHY This is the first study revealing that microRNA-23a (miR-23a) promotes hepatic fibrosis through the phosphatase and tensin homolog\/phosphatidylinositol 3-kinase\/protein kinase B\/mammalian target of rapamycin\/Snail signaling pathway, and long noncoding RNA (lncRNA) growth arrest-specific transcript 5 (GAS5) can act as a sponge platform for miR-23a. Therefore, lncRNA GAS5\/miR-23a may bring molecular targets for hepatic fibrosis therapy.<\/jats:p>","DOI":"10.1152\/ajpgi.00249.2018","type":"journal-article","created":{"date-parts":[[2019,2,8]],"date-time":"2019-02-08T14:23:12Z","timestamp":1549635792000},"page":"G539-G550","source":"Crossref","is-referenced-by-count":83,"title":["lncRNA GAS5 restrains CCl\n                    <sub>4<\/sub>\n                    -induced hepatic fibrosis by targeting miR-23a through the PTEN\/PI3K\/Akt signaling pathway"],"prefix":"10.1152","volume":"316","author":[{"given":"Zhiheng","family":"Dong","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner 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