{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:19:39Z","timestamp":1791155979482,"version":"4.1.0"},"reference-count":63,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:p>\n                    Inflammatory bowel diseases (IBD) are characterized during their active phase by polymorphonuclear leukocyte (PMNL) transepithelial migration. The efflux of PMNL into the mucosa is associated with the production of proinflammatory cytokines and the release of ATP from damaged and necrotic cells. The expression and function of purinergic P2X\n                    <jats:sub>7<\/jats:sub>\n                    receptor (P2X\n                    <jats:sub>7<\/jats:sub>\n                    R) in intestinal epithelial cells (IEC) and its potential role in the \u201ccross talk\u201d between IEC and PMNL have not been explored. The aims of the present study were 1) to examine P2X\n                    <jats:sub>7<\/jats:sub>\n                    R expression in IEC (T84 cells) and in human intestinal biopsies; 2) to detect any changes in P2X\n                    <jats:sub>7<\/jats:sub>\n                    R expression in T84 cells during PMNL transepithelial migration, and during the active and quiescent phases of IBD; and 3) to test whether P2X\n                    <jats:sub>7<\/jats:sub>\n                    R stimulation in T84 monolayers can induce caspase-1 activation and IL-1\u03b2 release by IEC. We found that a functional ATP-sensitive P2X\n                    <jats:sub>7<\/jats:sub>\n                    R is constitutively expressed at the apical surface of IEC T84 cells. PMNL transmigration regulates dynamically P2X\n                    <jats:sub>7<\/jats:sub>\n                    R expression and alters its distribution from the apical to basolateral surface of IEC during the early phase of PMNL transepithelial migration in vitro. P2X\n                    <jats:sub>7<\/jats:sub>\n                    R expression was weak in intestinal biopsies obtained during the active phase of IBD. We show that activation of epithelial P2X\n                    <jats:sub>7<\/jats:sub>\n                    R is mandatory for PMNL-induced caspase-1 activation and IL-1\u03b2 release by IEC. Overall, these changes in P2X\n                    <jats:sub>7<\/jats:sub>\n                    R function may serve to tailor the intensity of the inflammatory response and to prevent IL-1\u03b2 overproduction and inflammatory disease.\n                  <\/jats:p>","DOI":"10.1152\/ajpgi.00282.2009","type":"journal-article","created":{"date-parts":[[2010,2,25]],"date-time":"2010-02-25T22:18:19Z","timestamp":1267136299000},"page":"G32-G42","source":"Crossref","is-referenced-by-count":57,"title":["Amplification loop of the inflammatory process is induced by P2X\n                    <sub>7<\/sub>\n                    R activation in intestinal epithelial cells in response to neutrophil transepithelial migration"],"prefix":"10.1152","volume":"299","author":[{"given":"Annabelle","family":"Cesaro","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) ERI-21\/EA 4319, Nice;"},{"name":"Universit\u00e9 de Nice Sophia Antipolis, 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