{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T00:38:22Z","timestamp":1792197502119,"version":"4.4.0"},"reference-count":44,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:p> The role of Slc26a6 (PAT1) on apical Cl<jats:sup>\u2212<\/jats:sup>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> exchange and bicarbonate secretion in pancreatic duct cells was investigated using Slc26a6 null and wild-type (WT) mice. Apical Cl<jats:sup>\u2212<\/jats:sup>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> exchange activity was measured with the pH-sensitive dye BCECF in microperfused interlobular ducts. The HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>-influx mode of apical [Cl<jats:sup>\u2212<\/jats:sup>]<jats:sub>i<\/jats:sub>\/[HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>]<jats:sub>o<\/jats:sub> exchange (where brackets denote concentration and subscripts i and o denote intra- and extracellular, respectively) was dramatically upregulated in Slc26a6 null mice ( P &lt; 0.01 vs. WT), whereas the HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>-efflux mode of apical [Cl<jats:sup>\u2212<\/jats:sup>]<jats:sub>o<\/jats:sub>\/[HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>]<jats:sub>i<\/jats:sub> exchange was decreased in Slc26a6 null mice ( P &lt; 0.05 vs. WT), suggesting the unidirectionality of the Slc26a6-mediated HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> transport. Fluid secretory rate in interlobular ducts were comparable in WT and Slc26a6 null mice ( P &gt; 0.05). In addition, when pancreatic juice was collected from whole animal in basal and secretin-stimulated conditions, neither juice volume nor its pH showed differences between WT and Slc26a6 null mice. Semiquantitative RT-PCR demonstrated more than fivefold upregulation in Slc26a3 (DRA) expression in Slc26a6 knockout pancreas. In conclusion, these results point to the role of Slc26a6 in HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> efflux at the apical membrane and also suggest the presence of a robust Slc26a3 compensatory upregulation, which can replace the function of Slc26a6 in pancreatic ducts. <\/jats:p>","DOI":"10.1152\/ajpgi.00286.2006","type":"journal-article","created":{"date-parts":[[2006,8,11]],"date-time":"2006-08-11T00:34:21Z","timestamp":1155256461000},"page":"G447-G455","source":"Crossref","is-referenced-by-count":57,"title":["Effect of Slc26a6 deletion on apical Cl<sup>\u2212<\/sup>\/HCO<sub>3<\/sub><sup>\u2212<\/sup> exchanger activity and cAMP-stimulated bicarbonate secretion in pancreatic duct"],"prefix":"10.1152","volume":"292","author":[{"given":"Hiroshi","family":"Ishiguro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan","family":"Namkung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaohui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger T.","family":"Worrell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Goo","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoocher","family":"Soleimani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.64.092801.141759"},{"key":"R2","unstructured":"Alper SL, Darman RB, Chernova MN, Dahl NK. 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