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Rabbit slow waves<jats:sub>ICC<\/jats:sub>consisted of an upstroke depolarization followed by a distinct plateau component. Ni<jats:sup>2+<\/jats:sup>and nominally Ca<jats:sup>2+<\/jats:sup>-free solutions reduced the rate-of-rise and amplitude of the upstroke depolarization. Replacement of Ca<jats:sup>2+<\/jats:sup>with Sr<jats:sup>2+<\/jats:sup>enhanced the upstroke component but decreased the plateau component of rabbit slow waves<jats:sub>ICC<\/jats:sub>. In contrast, replacing Ca<jats:sup>2+<\/jats:sup>with Sr<jats:sup>2+<\/jats:sup>decreased both components of mouse slow waves<jats:sub>ICC<\/jats:sub>. The plateau component of rabbit slow waves<jats:sub>ICC<\/jats:sub>was inhibited in low-extracellular-Cl<jats:sup>\u2212<\/jats:sup>-concentration (low-[Cl<jats:sup>\u2212<\/jats:sup>]<jats:sub>o<\/jats:sub>) solutions and by 4,4\u2032-diisothiocyanostilbene-2,2\u2032-disulfonic acid (DIDS), an inhibitor of Cl<jats:sup>\u2212<\/jats:sup>channels, cyclopiazonic acid (CPA), an inhibitor of internal Ca<jats:sup>2+<\/jats:sup>pumps, or bumetanide, an inhibitor of Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>cotransporter (NKCC1). Bumetanide also inhibited the plateau component of mouse slow waves<jats:sub>ICC<\/jats:sub>. NKCC1-like immunoreactivity was observed mainly in ICC-MY in the rabbit small intestine. Membrane depolarization with a high-K<jats:sup>+<\/jats:sup>solution reduced the upstroke component of rabbit slow waves<jats:sub>ICC<\/jats:sub>. In cells depolarized with elevated external K<jats:sup>+<\/jats:sup>, DIDS, CPA, and bumetanide blocked slow waves<jats:sub>ICC<\/jats:sub>. These results suggest that the upstroke component of rabbit slow waves<jats:sub>ICC<\/jats:sub>is partially mediated by voltage-dependent Ca<jats:sup>2+<\/jats:sup>influx, whereas the plateau component is dependent on Ca<jats:sup>2+<\/jats:sup>-activated Cl<jats:sup>\u2212<\/jats:sup>efflux. NKCC1 is likely to be responsible for Cl<jats:sup>\u2212<\/jats:sup>accumulation in ICC-MY. The results also suggest that the mechanism of the upstroke component differs in rabbit and mouse slow waves<jats:sub>ICC<\/jats:sub>in the small intestine.<\/jats:p>","DOI":"10.1152\/ajpgi.00308.2014","type":"journal-article","created":{"date-parts":[[2014,12,25]],"date-time":"2014-12-25T05:56:51Z","timestamp":1419487011000},"page":"G378-G388","source":"Crossref","is-referenced-by-count":25,"title":["Characterization of slow waves generated by myenteric interstitial cells of Cajal of the rabbit small intestine"],"prefix":"10.1152","volume":"308","author":[{"given":"Yoshihiko","family":"Kito","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, Saga University, Nabeshima, Saga, Japan;"},{"name":"Department of Cell Physiology, Nagoya City University Medical School, Mizuho-ku, Nagoya, Japan; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Retsu","family":"Mitsui","sequence":"additional","affiliation":[{"name":"Department of Cell 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