{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T14:31:53Z","timestamp":1777559513095,"version":"3.51.4"},"reference-count":21,"publisher":"American Physiological Society","issue":"1","funder":[{"DOI":"10.13039\/501100001700","name":"Japanese Ministry of Education, Science, Sports and Culture","doi-asserted-by":"crossref","award":["15K21037"],"award-info":[{"award-number":["15K21037"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Japan Dairy Association","award":["J-milk"],"award-info":[{"award-number":["J-milk"]}]},{"name":"Japan Dairy Association","award":["2016"],"award-info":[{"award-number":["2016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2019,1,1]]},"abstract":"<jats:p> The traditional Japanese health care custom recommends that a suitable volume of water is consumed. However, physiological and immunological mechanisms in support of this practice are unknown. Therefore, we conducted rat and rabbit in vivo experiments to investigate the effects of intragastric administration of distilled water on the jejunal-originated lymph flow and the concentrations and total flux of cells, albumin, long-chain fatty acids, and innate lymphoid cell 3 (ILC-3)-secreted interleukin-22 (IL-22) through mesenteric lymph vessels. The distribution and activity of ILC-3 in rat small intestine by water intake were evaluated using flow cytometry and RT-PCR. The intragastric administration of distilled water caused significant increases in rat mesenteric lymph flow and in the total flux of cells, albumin, long-chain fatty acids, and IL-22 through the lymph vessels. Intravenously injected Evans blue dye was rapidly transported into rabbit mesenteric lymph vessel and cisterna chyli. The distribution of ILC-3 and the expression of IL-22 mRNA were maximal in the lamina propria cells of the rat jejunum. No significant presence of ILC-3 in the lymph was observed in the control and under water intake conditions. In conclusion, the absorbed water in the jejunum is transported through mesenteric lymph vessels. The higher permeability of albumin in the jejunal microcirculation may play key roles in the transport of consumed water and the reservoir and transporter of long-chain fatty acids. Water intake also accelerates the transfer of IL-22 to the mesenteric lymph, which may contribute, in part, to maintaining and promoting the innate immunity in the body. <\/jats:p><jats:p> NEW &amp; NOTEWORTHY The higher permeability of albumin-mediated transport of water-soluble substances in mesenteric lymph vessels of the jejunum may have a large impact on the classic concept suggesting that water-soluble small molecules travel to the liver via the portal vein. ILC-3 is mainly housed in the lamina propria of the jejunum, especially its upper part. IL-22 released from the ILC-3 is also transported through mesenteric lymph in collaboration with the albumin-mediated movement of consumed water. <\/jats:p>","DOI":"10.1152\/ajpgi.00325.2018","type":"journal-article","created":{"date-parts":[[2018,11,15]],"date-time":"2018-11-15T11:04:12Z","timestamp":1542279852000},"page":"G155-G165","source":"Crossref","is-referenced-by-count":18,"title":["Water intake increases mesenteric lymph flow and the total flux of albumin, long-chain fatty acids, and IL-22 in rats: new concept of absorption in jejunum"],"prefix":"10.1152","volume":"316","author":[{"given":"Sachiho","family":"Nagashio","sequence":"first","affiliation":[{"name":"Department of Innovation of Medical and Health Sciences Research, Shinshu University School of Medicine, Matsumoto, Japan"},{"name":"Department of Dentistry and Oral Surgery, Shinshu University School of Medicine, Matsumoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumiko","family":"Ajima","sequence":"additional","affiliation":[{"name":"Department of Innovation of Medical and Health Sciences Research, Shinshu University School of Medicine, Matsumoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Maejima","sequence":"additional","affiliation":[{"name":"Department of Innovation of Medical and Health Sciences Research, Shinshu University School of Medicine, Matsumoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideki","family":"Sanjo","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Immunology, Shinshu University School of Medicine, Matsumoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryo","family":"Kajihara","sequence":"additional","affiliation":[{"name":"Department of Innovation of Medical and Health Sciences Research, Shinshu University 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