{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:08:24Z","timestamp":1773299304013,"version":"3.50.1"},"reference-count":36,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:p>This study was designed to clarify whether mitochondrial function\/dysfunction and reactive oxygen species (ROS) production have a temporal relationship with organ failure during endotoxic shock. Adult male Sprague-Dawley rats were divided into three groups receiving 1) isotonic saline (control group, n = 16); 2) 8 mg\/kg lipopolysaccharide (LPS; n = 8); or 3) 20 mg\/kg LPS ( n = 8) intraperitoneally under short anesthesia with 3.5% of isoflurane. After 16 h, animals were killed to analyze plasma, rat liver mitochondria (RLM), and rat heart mitochondria (RHM). In accordance with plasma analysis, LPS-treated rats were divided into \u201cresponders\u201d and \u201cnonresponders\u201d with high and low levels of alanine aminotransferase and creatine, respectively. RHM from responders had significantly lower respiratory activity in state 3, suggesting a decreased rate of ATP synthesis. In contrast, RLM from responders had significantly higher respiratory activity in state 3 than both nonresponders and the control group. This increase was accompanied by a decrease in phosphate-to-oxygen ratio values, which was not observed in RHM. ROS generation determined with a spin probe, 1-hydroxy-3-carboxypyrrolidine, neither revealed a difference in RHM between LPS and control groups nor between responders and nonresponders. In contrast, RLM isolated from responders showed a marked increase in ROS production compared with both the control group and nonresponders. Our data demonstrate that 1) RHM and RLM respond to endotoxic shock in a different manner, decreasing and increasing respiratory activity, respectively, and 2) there is a temporal relationship between ROS production in RLM (but not in RHM) and tissue damage in rats subjected to LPS shock.<\/jats:p>","DOI":"10.1152\/ajpgi.00331.2005","type":"journal-article","created":{"date-parts":[[2006,2,10]],"date-time":"2006-02-10T19:47:51Z","timestamp":1139600871000},"page":"G543-G549","source":"Crossref","is-referenced-by-count":46,"title":["Different effects of endotoxic shock on the respiratory function of liver and heart mitochondria in rats"],"prefix":"10.1152","volume":"290","author":[{"given":"Andrey V.","family":"Kozlov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katrin","family":"Staniek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Haindl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Piskernik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"\u00d6hlinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Gille","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"Nohl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soheyl","family":"Bahrami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinz","family":"Redl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(03)02037-5"},{"key":"R2","doi-asserted-by":"crossref","unstructured":"Ben Shaul V, Lomnitski L, Nyska A, Zurovsky Y, Bergman M, and Grossman S.The effect of natural antioxidants, NAO and apocynin, on oxidative stress in the rat heart following LPS challenge.Toxicol Lett123: 1\u201310, 2001.","DOI":"10.1016\/S0378-4274(01)00369-1"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1042\/bj3150295"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-003-2121-4"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000110671.47539.03"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-200202000-00002"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000109449.99160.81"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6174"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1006\/niox.1997.0139"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-200208000-00012"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4804(86)90225-8"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1998.0699"},{"key":"R13","unstructured":"Houtchens BAand Westenskow DR.Oxygen consumption in septic shock: collective review.Circ Shock13: 361\u2013384, 1984."},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1997.0264"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(03)00179-5"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702123"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015921513720"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1023\/A:1027328510931"},{"key":"R19","unstructured":"Lu SM, Song SM, Liu JC, Yang HM, Li P, and Wang ZG.Changes of proton transportation across the inner mitochondrial membrane and H+-ATPase in endotoxic shock rats.Chin J Traumatol6: 292\u2013296, 2003."},{"key":"R20","doi-asserted-by":"crossref","unstructured":"Mela L, Bacalzo LV Jr, and Miller LD.Defective oxidative metabolism of rat liver mitochondria in hemorrhagic and endotoxin shock.Am J Physiol220: 571\u2013577, 1971.","DOI":"10.1152\/ajplegacy.1971.220.2.571"},{"key":"R21","unstructured":"Mela-Riker L, Bartos D, Vlessis AA, Widener L, Muller P, and Trunkey DD.Chronic hyperdynamic sepsis in the rat. 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