{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,12]],"date-time":"2025-04-12T23:10:25Z","timestamp":1744499425710},"reference-count":35,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:p>In guinea pig antral mucous cells, ACh stimulates the Ca<jats:sup>2+<\/jats:sup>-regulated exocytosis, which has a characteristics feature: an initial transient phase followed by a sustained phase. The effects of cGMP on ACh-stimulated exocytosis were studied in guinea pig antral mucous cells using video microscopy. cGMP enhanced the frequency of ACh-stimulated exocytotic events, whereas cGMP alone did not induce any exocytotic events under the ACh-unstimulated condition. cGMP did not stimulate either Ca<jats:sup>2+<\/jats:sup>mobilization or cAMP accumulation. The Ca<jats:sup>2+<\/jats:sup>dose-response studies demonstrated that cGMP shifted the dose-response curve upward with no shift to the lower concentration. This indicates that cGMP increased maximal responsiveness of the Ca<jats:sup>2+<\/jats:sup>-regulated exocytosis, but not the Ca<jats:sup>2+<\/jats:sup>sensitivity. Moreover, under a condition of ATP depletion by dinitrophenol (DNP) or anoxia (N<jats:sub>2<\/jats:sub>bubbling), ACh evoked only a sustained phase in exocytotic events with no initial transient phase. However, ACh evoked an initial transient phase followed by a sustained phase with addition of cGMP before ATP depletion, whereas only a sustained phase was evoked in a case of cGMP addition after ATP depletion. Thus cGMP-induced enhancement in ACh-stimulated exocytotic events requires ATP, suggesting that cGMP modulates ATP-dependent priming of Ca<jats:sup>2+<\/jats:sup>-regulated exocytosis in antral mucous cells. In conclusion, cGMP increases the number of primed granules via acceleration of the ATP-dependent priming, which enhances the Ca<jats:sup>2+<\/jats:sup>-regulated exocytosis stimulated by ACh.<\/jats:p>","DOI":"10.1152\/ajpgi.00357.2005","type":"journal-article","created":{"date-parts":[[2006,1,20]],"date-time":"2006-01-20T01:40:19Z","timestamp":1137721219000},"page":"G1138-G1148","source":"Crossref","is-referenced-by-count":10,"title":["cGMP modulation of ACh-stimulated exocytosis in guinea pig antral mucous cells"],"prefix":"10.1152","volume":"290","author":[{"given":"Adel H.","family":"Saad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chikao","family":"Shimamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Nakahari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoko","family":"Fujiwara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken-ichi","family":"Katsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinori","family":"Marunaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Bahnson TD, Pandol SJ, and Dionne VE.Cyclic GMP modulates depletion-activated Ca2+entry in pancreatic acinar cells.J Biol Chem268: 10808\u201310812, 1993.","DOI":"10.1016\/S0021-9258(18)82057-9"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(95)90071-3"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1042\/bj3230521"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.00317.x"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1994.tb17009.x"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.085aa.x"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2005.032482"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1159\/000007678"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1994.266.3.G350"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2004.028746"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2003.026617"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.301.1.355"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(93)90639-Y"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2004.028670"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1991.tb12457.x"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(88)83045-5"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000930200"},{"key":"R18","unstructured":"Maurice DHand Haslam RJ.Molecular basis of the synergistic inhibition of platelet function by nitrovasodilators and activators of adenylate cyclase: inhibition of cyclic AMP breakdown by cyclic GMP.Mol Pharmacol37: 671\u2013681, 1990."},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1016\/S1054-3589(08)61091-1"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2004.028837"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00300.2001"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1113\/eph8602160"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1997.273.3.G655"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.25.12086"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)00480-8"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.32.18781"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90267-4"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00060.2004"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1998.274.6.G1166"},{"key":"R30","unstructured":"Waldman SAand Murad F.Cyclic GMP synthesis and function.Pharmacol Rev39: 163\u2013196, 1987."},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.3.C557"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1159\/000201524"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4160(97)90015-4"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050359"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(98)00007-X"}],"container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.00357.2005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T21:47:44Z","timestamp":1626990464000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.00357.2005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,6]]},"references-count":35,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,6]]}},"alternative-id":["10.1152\/ajpgi.00357.2005"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.00357.2005","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,6]]}}}