{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T17:50:29Z","timestamp":1757699429614},"reference-count":57,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:p> By chorda tympani denervation (CTD, parasympathectomy), the aquaporin 5 (AQP5), but not AQP1, protein level in the rat submandibular gland (SMG) was significantly decreased, dropping to 37% of that of the contralateral gland at 4 wk. The protein levels of AQP5 and AQP1 were not significantly affected by denervation of the cervical sympathetic trunk (sympathectomy). Administration of cevimeline hydrochloride, an M3 muscarinic receptor agonist (10 mg\/kg for 7 days po), but not pilocarpine (0.3 mg\/kg for 7 days po), recovered the AQP5 protein level reduced by CTD and increased the AQP1 protein level above the control one. The mRNA level of AQP5 was scarcely affected by CTD and cevimeline hydrochloride administration. Administration of chloroquine (50 mg\/kg for 7 days po), a denaturant of lysosomes, increased the AQP5 protein level reduced by CTD. An extract obtained from the submandibular lysosomal fraction degraded the AQP5 protein in the total membrane fraction in vitro. These results suggest the possible regulation of the AQP5 protein level in the SMG by the parasympathetic nerves\/M3 muscarinic receptor agonist and imply the involvement of lysosomal enzymes, but not a transcriptional mechanism, in this regulation. <\/jats:p>","DOI":"10.1152\/ajpgi.00359.2007","type":"journal-article","created":{"date-parts":[[2008,5,3]],"date-time":"2008-05-03T21:48:49Z","timestamp":1209851329000},"page":"G112-G123","source":"Crossref","is-referenced-by-count":23,"title":["Degradation of submandibular gland AQP5 by parasympathetic denervation of chorda tympani and its recovery by cevimeline, an M3 muscarinic receptor agonist"],"prefix":"10.1152","volume":"295","author":[{"given":"Xuefei","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmad","family":"Azlina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mileva Ratko","family":"Karabasil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nunuk","family":"Purwanti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiro","family":"Hasegawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenjuan","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Akamatsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Hosoi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-003-1109-9"},{"key":"R2","unstructured":"Agre P. Molecular physiology of water transport: aquaporin nomenclature workshop. Mammalian aquaporins. Biol Cell 89: 225\u2013257, 1997."},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.24.14659"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2004.10.005"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00316.2004"},{"key":"R6","unstructured":"Bloom FE. Neurotransmission and the central nervous system, In: Goodman & Gilman's The Pharmacological Basis of Therapeutics (10th edition), edited by Hardman JG and Limbird LE (consulting editor, Gilman AG). New York: McGraw-Hill, 2001, chapt. 12, p. 293\u2013320."},{"key":"R7","unstructured":"Bradley RM. (editor) Salivary secretion. In: Essentials of Oral Physiology. St. Louis, MO: Mosby-Year, 1995, p. 161\u2013186."},{"key":"R8","unstructured":"Brown JH, Taylor P. Muscarinic agonist and antagonist. In: Goodman & Gilman's The Pharmacological Basis of Therapeutics (10th edition), edited by Hardman JG and Limbird LE (consulting editor, Gilman AG). New York: McGraw-Hill, 2001, chapt. 7, p. 155\u2013173."},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00386.2006"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1126\/science.8140421"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1038\/361549a0"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.33.20782"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8252"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.7219"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(02)00393-7"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.14.6269"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.03.046"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1016\/0306-3623(94)90021-3"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2004.01.011"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.26.13052"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0604073103"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.273.5.C1541"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.48.30329"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050731"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(90)90191-H"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1159\/000092918"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.041616498"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1007\/BF00374565"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-007-0399-1"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.29.20071"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(07)70076-5"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1007\/s004410051257"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.63.1.607"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-002-0940-8"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00449.2005"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.24.11663"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00030.2004"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(01)00172-0"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.24.11110"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1126\/science.256.5055.385"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.4.1908"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.60.1.199"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411038200"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0463.2002.100606.x"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1828"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"R47","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100322200"},{"key":"R48","doi-asserted-by":"publisher","DOI":"10.1042\/bj2980079"},{"key":"R49","doi-asserted-by":"crossref","unstructured":"Tsukahara T, Ishiura S, Sugita H. An ATP-dependent protease and ingensin, the multicatalytic proteinase, in K562 cells. Eur J Biochem 177: 261\u2013266, 1998.","DOI":"10.1111\/j.1432-1033.1988.tb14371.x"},{"key":"R50","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(84)90049-6"},{"key":"R51","doi-asserted-by":"publisher","DOI":"10.1038\/42512"},{"key":"R52","unstructured":"Xie YP, Chen CP, Wang JC, Qian GS, Wang YD, Xiao ZL. Experimental study on the expression and function of aquaporin-1 and aquaporin-5 in rats with acute lung injury induced by lipopolysaccharide. Zhonghua Jie He He Hu Xi Za Zhi 28: 385\u2013389, 2005."},{"key":"R53","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M305149200"},{"key":"R54","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M507705200"},{"key":"R55","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2005.02212.x"},{"key":"R56","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a022396"},{"key":"R57","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00070.2006"}],"container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.00359.2007","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T21:27:42Z","timestamp":1567978062000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.00359.2007"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7]]},"references-count":57,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,7]]}},"alternative-id":["10.1152\/ajpgi.00359.2007"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.00359.2007","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7]]}}}