{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:31:38Z","timestamp":1792787498601,"version":"4.5.0"},"reference-count":31,"publisher":"American Physiological Society","issue":"4","funder":[{"DOI":"10.13039\/501100005046","name":"the Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"crossref","award":["No. H2017078"],"award-info":[{"award-number":["No. H2017078"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Biomedicine Climbing Project for Provincial Colleges of Department of Education, Heilongjiang Province","award":["No. 2017-KYYWFMY-0653 and 2017-KYYWFMY-0684"],"award-info":[{"award-number":["No. 2017-KYYWFMY-0653 and 2017-KYYWFMY-0684"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2020,4,1]]},"abstract":"<jats:p>There is increasing evidence that microRNA (miRNA) abnormity is involved in the occurrence and the development of various malignancies, including colon cancer. MiRNA-524\u20135p has been reported to possess anticancer activity in various tumors, which function is seldom investigated in colon cancer cells. The aim of this study was to explore the effect of the miRNA-524\u20135p\/with-no-lysine kinase 1 (WNK1) system on angiogenesis in a colon cancer cell line (HT-29 and COLO205 cells) and further investigate the potential mechanisms. We found miRNA-524\u20135p expression was relatively high in COLO205 cells and relatively low in HT-29 cells. Elevating miRNA-524\u20135p expression inhibited proliferation, induced cycle arrest, diminished vascular endothelial growth factor production, and thereby suppressed angiogenesis in HT-29 cells. WNK1 silencing exerted the ability of antiangiogenesis in HT-29 cells. Besides, miRNA-524\u20135p deficiency-induced angiogenesis was impeded by WNK1 silence in COLO205 cells. In a murine tumor model, miRNA-524\u20135p agomir treatment significantly suppressed colon cancer tumorigenicity with the downregulation of WNK1 expression. In summary, our results indicated that miRNA-524\u20135p inhibited angiogenesis in colon cancer cells via targeting WNK1.<\/jats:p>\n                  <jats:p>NEW &amp; NOTEWORTHY MiRNA-524\u20135p inhibited angiogenesis in colon cancer cells via targeting with-no-lysine kinase 1.<\/jats:p>","DOI":"10.1152\/ajpgi.00369.2019","type":"journal-article","created":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T07:14:01Z","timestamp":1584342841000},"page":"G827-G839","source":"Crossref","is-referenced-by-count":26,"title":["miR-524-5p inhibits angiogenesis through targeting WNK1 in colon cancer cells"],"prefix":"10.1152","volume":"318","author":[{"given":"Xiang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Ultrasonic Diagnosis, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, People\u2019s Republic of 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