{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:36:45Z","timestamp":1791121005023,"version":"4.1.0"},"reference-count":51,"publisher":"American Physiological Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2013,6,15]]},"abstract":"<jats:p>Hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) affects vascular resistance; however, its effect on the hepatic microcirculation has not been investigated. Hepatic sinusoidal perfusion is dysregulated during sepsis, contributing to liver injury. Therefore, the present study determined the effect of H<jats:sub>2<\/jats:sub>S on the hepatic microcirculation and the contribution of endogenous H<jats:sub>2<\/jats:sub>S to hepatic microcirculatory dysfunction in an endotoxin model of sepsis. Portal infusion of H<jats:sub>2<\/jats:sub>S increased portal pressure in vivo (6.8 \u00b1 0.2 mmHg before H<jats:sub>2<\/jats:sub>S vs. 8.6 \u00b1 0.8 mmHg peak during H<jats:sub>2<\/jats:sub>S infusion, P &lt; 0.05). Using intravital microscopy, we observed decreased sinusoidal diameter (6.2 \u00b1 0.27 \u03bcm before H<jats:sub>2<\/jats:sub>S vs. 5.7 \u00b1 0.3 \u03bcm after H<jats:sub>2<\/jats:sub>S, P &lt; 0.05) and increased sinusoidal heterogeneity during H<jats:sub>2<\/jats:sub>S infusion ( P &lt; 0.05) and net constriction. Since hepatic H<jats:sub>2<\/jats:sub>S levels are elevated during sepsis, we used the cystathionine \u03b3 lyase inhibitor dl-propargylglycine (PAG) to determine the contribution of H<jats:sub>2<\/jats:sub>S to the hypersensitization of the sinusoid to the vasoconstrictor effect of endothelin-1 (ET-1). PAG treatment significantly attenuated the sinusoidal sensitization to ET-1 in endotoxin-treated animals. ET-1 infusion increased portal pressure to 175% of baseline in endotoxemic animals, which was reduced to 143% following PAG treatment ( P &lt; 0.05). PAG abrogated the increase in sinusoidal constriction after ET-1 infusion in LPS-treated rats (30.9% reduction in LPS rats vs. 11.6% in PAG\/LPS rats, P &lt; 0.05). Moreover, PAG treatment significantly attenuated the increase in NADH fluorescence following ET-1 exposure during endotoxemia (61 grayscale units LPS vs. 21 units in PAG\/LPS, P &lt; 0.05), suggesting an improvement in hepatic oxygen availability. This study is the first to demonstrate a vasoconstrictor action of H<jats:sub>2<\/jats:sub>S on the hepatic sinusoid and provides a possible mechanism for the protective effect of PAG treatment during sepsis.<\/jats:p>","DOI":"10.1152\/ajpgi.00395.2012","type":"journal-article","created":{"date-parts":[[2013,3,22]],"date-time":"2013-03-22T02:09:42Z","timestamp":1363918182000},"page":"G1070-G1078","source":"Crossref","is-referenced-by-count":24,"title":["Hydrogen sulfide modulates sinusoidal constriction and contributes to hepatic micorcirculatory dysfunction during endotoxemia"],"prefix":"10.1152","volume":"304","author":[{"given":"Eric J.","family":"Norris","sequence":"first","affiliation":[{"name":"Department of Biology, University of North Carolina at Charlotte, Charlotte, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Feilen","sequence":"additional","affiliation":[{"name":"Department of Biology, University of North Carolina at 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