{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T23:54:58Z","timestamp":1787702098687,"version":"build-2784847793"},"reference-count":37,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:p>Facilitative UT-B urea transporters enable the passage of urea across cell membranes. Gastrointestinal urea transporters are thought to play a significant role in the urea nitrogen salvaging process that occurs between mammalian hosts and their gut bacteria. This study investigated the expression of UT-B urea transporters in different segments of human colon. Immunoblot analysis showed that human colon expressed a 35-kDa glycosylated UT-B protein in the colonic mucosa. The 35-kDa UT-B transporter was predominantly located in plasma membrane-enriched samples ( P &lt; 0.001; n = 6), and its expression was greater in the ascending colon compared with the descending colon ( P &lt; 0.01; n = 3). At the cellular level, UT-B transporters were located throughout colonocytes situated in the upper portion of the colonic crypts. Bidirectional trans-epithelial urea transport was significantly greater in the ascending colon than the descending colon ( P &lt; 0.05; n = 6). In addition, the facilitative urea transporter inhibitor 1,3,dimethylurea significantly reduced urea transport in the ascending colon ( P &lt; 0.05; n = 6) but had no effect in the descending colon (NS; n = 6). These results illustrate differential protein abundance of functional UT-B protein in different sections of the human colon, strongly correlating to regions that contain the largest populations of intestinal bacteria. This study suggests an important role for UT-B urea transporters in maintaining the symbiotic relationship between humans and their gut bacteria.<\/jats:p>","DOI":"10.1152\/ajpgi.00405.2009","type":"journal-article","created":{"date-parts":[[2009,11,20]],"date-time":"2009-11-20T03:14:29Z","timestamp":1258686869000},"page":"G345-G351","source":"Crossref","is-referenced-by-count":31,"title":["Differential protein abundance and function of UT-B urea transporters in human colon"],"prefix":"10.1152","volume":"298","author":[{"given":"D.","family":"Collins","sequence":"first","affiliation":[{"name":"Institute for Clinical Outcomes Research and Education, St. Vincent's University Hospital;"},{"name":"College of Life Sciences and Conway Institute of Biomedical and Bimolecular Science; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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