{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T04:06:23Z","timestamp":1747368383807,"version":"3.40.5"},"reference-count":30,"publisher":"American Physiological Society","issue":"5","funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"crossref","award":["MOP12874"],"award-info":[{"award-number":["MOP12874"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2015,3,1]]},"abstract":"<jats:p>Cannon described in 1902 the segmentation motor activity of the small intestine (Canon WB. J Med Res 7: 72\u201375, 1902). This motor pattern can arise when low-frequency transient depolarizations are evoked in the interstitial cells of Cajal associated with the deep muscular plexus (ICC-DMP) network, which then affect the omnipresent slow wave activity: changing its regular amplitude into a waxing and waning pattern. The objective of the present study was to investigate physiological stimuli that could induce the low-frequency component. Intracellular recordings were obtained from circular muscle with or without attached mucosa. Decanoic acid (1 mM) and butyric acid (10 mM) both evoked low-frequency transient depolarizations but through different mechanisms. Decanoic acid-induced waxing and waning was initiated by purely myogenic means when perfused onto exposed circular muscle. Butyric acid required the intact mucosa and uninhibited neural activity to elicit the low-frequency response. Evidence is provided that the transient rhythmic depolarizations occur in the absence of interstitial cells of Cajal associated with the myenteric plexus (ICC-MP). Onset of the slow transient depolarizations was stimulated by addition of N<jats:sup>\u03c9<\/jats:sup>-nitro-l-arginine (l-NNA; 100 \u03bcM); thus the low-frequency component seems to be under chronic inhibition by nitric oxide. Excitatory tachykinergic stimulation induced the low-frequency component since substance P (0.5 \u03bcM) evoked it in the presence of neural blockade. In summary, interplay between two networks of myogenic pacemakers, neural activity, and nutrient factors such as fatty acids plays a role in the generation of the rhythmic low-frequency component that is essential for the development of the checkered segmentation motor pattern.<\/jats:p>","DOI":"10.1152\/ajpgi.00409.2014","type":"journal-article","created":{"date-parts":[[2014,12,25]],"date-time":"2014-12-25T05:56:51Z","timestamp":1419487011000},"page":"G427-G433","source":"Crossref","is-referenced-by-count":10,"title":["Induction of rhythmic transient depolarizations associated with waxing and waning of slow wave activity in intestinal smooth muscle"],"prefix":"10.1152","volume":"308","author":[{"given":"Andrew J.","family":"Pawelka","sequence":"first","affiliation":[{"name":"Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan D.","family":"Huizinga","sequence":"additional","affiliation":[{"name":"Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.21982"},{"key":"B2","first-page":"72","volume":"7","author":"Cannon WB","year":"1902","journal-title":"J Med Res"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.2174\/1567201052772834"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/j.tics.2010.09.001"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0060474"},{"key":"B6","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1152\/ajplegacy.1969.216.2.301","volume":"216","author":"Diamant NE","year":"1969","journal-title":"Am J Physiol"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00358.2012"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2006.tb00416.x"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1017\/S0954422410000247"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00441.2006"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4326"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2009.00686.x"},{"key":"B13","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/S0022-3565(24)37216-7","volume":"284","author":"Lindmark T","year":"1998","journal-title":"J Pharmacol Exp Ther"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.3109\/10611869808995876"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1139\/y94-012"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1993.264.1.G64"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1994.266.3.G485"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.280.3.G439"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-014-1500-8"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12393"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00338.2014"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.281.1.G255"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1986.250.1.G28"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1990.258.6.G992"},{"key":"B25","first-page":"127","volume-title":"Smooth Muscle; an Assessment of Current Knowledge","author":"Tomita T","year":"1981"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19970630)383:2<153::AID-CNE3>3.0.CO;2-#"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.105189"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.t01-1-00355.x"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1988.255.6.G800"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-013-1374-1"}],"container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.00409.2014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T13:12:23Z","timestamp":1747314743000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.00409.2014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,1]]},"references-count":30,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,3,1]]}},"alternative-id":["10.1152\/ajpgi.00409.2014"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.00409.2014","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"type":"print","value":"0193-1857"},{"type":"electronic","value":"1522-1547"}],"subject":[],"published":{"date-parts":[[2015,3,1]]}}}