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Expression of NO synthase (NOS) isoforms was examined by real-time RT-PCR of ileal mucosa from control and C. parvum-infected piglets. The isoforms mediating and mechanism of NO action on barrier function were assessed by measuring transepithelial resistance (TER) and eicosanoid synthesis by ileal mucosa mounted in Ussing chambers in the presence of selective and nonselective NOS inhibitors and after rescue with exogenous prostaglandins. C. parvum infection results in induction of mucosal inducible NOS (iNOS), increased synthesis of NO and PGE<jats:sub>2<\/jats:sub>, and increased mucosal permeability. Nonselective inhibition of NOS ( N<jats:sup>G<\/jats:sup>-nitro-l-arginine methyl ester) inhibited prostaglandin synthesis, resulting in further increases in paracellular permeability. Baseline permeability was restored in the absence of NO by exogenous PGE<jats:sub>2<\/jats:sub>. Selective inhibition of iNOS [l- N<jats:sup>6<\/jats:sup>-(1-iminoethyl)-l-lysine] accounted for \u223c50% of NOS-dependent PGE<jats:sub>2<\/jats:sub>synthesis and TER. Using an entire intestinal mucosa, we have demonstrated for the first time that NO serves as a proximal mediator of PGE<jats:sub>2<\/jats:sub>synthesis and barrier function in C. parvum infection. Expression of iNOS by infected mucosa was without detriment to overall barrier function and may serve to promote clearance of infected enterocytes.<\/jats:p>","DOI":"10.1152\/ajpgi.00413.2003","type":"journal-article","created":{"date-parts":[[2004,5,25]],"date-time":"2004-05-25T00:43:45Z","timestamp":1085445825000},"page":"G571-G581","source":"Crossref","is-referenced-by-count":38,"title":["Nitric oxide synthase stimulates prostaglandin synthesis and barrier function in<i>C. parvum<\/i>-infected porcine ileum"],"prefix":"10.1152","volume":"287","author":[{"given":"Jody L.","family":"Gookin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurel L.","family":"Duckett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha U.","family":"Armstrong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen 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