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In the present study we investigated the very early effects of DNBS-ethanol colitis on ileal enterocyte TJ proteins [occludin, zonula occludens-1 (ZO-1), claudin-2] in controls, mice treated with infliximab (IFX) or with etanercept (ETC), and in knockout mice for the TNF-\u03b1 receptor 1 (TNFR-1<jats:sup>\u2212\/\u2212<\/jats:sup>). Circulating TNF-\u03b1 levels were effectively reduced by IFX and ETC ( P &lt; 0.01, both) at 3 and at 6 h. DNBS colitis induced disappearance of occludin and ZO-1 from enterocyte cell-cell contact, whereas claudin-2, absent under control conditions, appeared in the ileal epithelium. These alterations were prevented equally by both treatments, IFX and ETC, and in TNFR-1<jats:sup>\u2212\/\u2212<\/jats:sup> animals. DNBS colitis induced a very rapid loss of occludin and ZO-1 from ileal TJ together with an upregulation of claudin-2. Our data are consistent with the hypothesis that TNF-\u03b1 is involved in early TJ rearrangement and that its effects are mediated through TNFR-1. Despite clinical differences, both anti-TNF treatments were equally effective in the present setting. <\/jats:p>","DOI":"10.1152\/ajpgi.00469.2007","type":"journal-article","created":{"date-parts":[[2008,2,8]],"date-time":"2008-02-08T02:22:08Z","timestamp":1202437328000},"page":"G938-G947","source":"Crossref","is-referenced-by-count":47,"title":["Dynamics of enterocyte tight junctions: effect of experimental colitis and two different anti-TNF strategies"],"prefix":"10.1152","volume":"294","author":[{"given":"Walter","family":"Fries","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmelo","family":"Muja","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmela","family":"Crisafulli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Cuzzocrea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela","family":"Mazzon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00165"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700399"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1326.018"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1172\/JCI29218"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(99)70005-3"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20189"},{"key":"R7","unstructured":"Furuse M, Fujita K, Hiiragi T, Fujimoto K, Tsukita S. 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