{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T20:37:39Z","timestamp":1762375059378},"reference-count":52,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:p>Hemorrhagic shock and resuscitation cause endotoxemia and hepatocellular damage. Because lipopolysaccharide-binding protein (LBP) enhances cellular responses to endotoxin, our aim was to determine whether LBP contributes to hemorrhage\/resuscitation-induced injury by comparing LBP knockout and wild-type mice. Under pentobarbital anaesthesia, wild-type and LBP-deficient mice were hemorrhaged to 30 mmHg for 3 h and then resuscitated with shed blood plus half the volume of lactated Ringer solution. Serum alanine aminotransferase (ALT) necrosis, neutrophil infiltration, and 4-hydroxynonenal by histology\/cytochemistry and stress kinase activation by immunoblot analysis were then determined. ALT in wild-type mice was 2,461 \u00b1 383 and 1,418 \u00b1 194 IU\/l (means \u00b1 SE), respectively, at 2 and 6 h after resuscitation versus sham ALT of 102 \u00b1 6 IU\/l. In LBP-deficient mice, ALT was blunted at both time points to 1,108 \u00b1 340 and 619 \u00b1 171 IU\/l ( P &lt; 0.05). Liver necrosis after 6 h was also attenuated from 3.5 \u00b1 0.8% in wild-type mice to 1.3 \u00b1 0.5% in LBP-deficient mice ( P &lt; 0.05). After hemorrhage\/resuscitation, neutrophil infiltration increased 71% more in wild-type than LBP knockout mice. Similarly, hepatic 4-hydroxynonenal staining, indicative of lipid peroxidation, decreased from 33.8 \u00b1 4.5% in wild-type mice to 11.6 \u00b1 1.9% in knockout mice ( P &lt; 0.05). After hemorrhage\/resuscitation, activation of MAPKs, JNK and ERK, occurred in wild-type mice, which was largely blocked in LBP-deficient mice. However, endotoxin in portal blood after resuscitation was not significantly different between wild-type and knockout mice. In conclusion, hemorrhagic shock and resuscitation to mice cause severe, LBP-mediated hepatocellular damage. An absence of LBP blunts hepatocellular injury with decreased neutrophil infiltration, oxidative stress, and c-Jun and ERK activation.<\/jats:p>","DOI":"10.1152\/ajpgi.00480.2005","type":"journal-article","created":{"date-parts":[[2006,4,14]],"date-time":"2006-04-14T01:25:48Z","timestamp":1144977948000},"page":"G456-G463","source":"Crossref","is-referenced-by-count":21,"title":["Lipopolysaccharide-binding protein modulates hepatic damage and the inflammatory response after hemorrhagic shock and resuscitation"],"prefix":"10.1152","volume":"291","author":[{"given":"Mark","family":"Lehnert","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Uehara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Blair U.","family":"Bradford","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrik","family":"Lind","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Brenner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingo","family":"Marzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John J.","family":"Lemasters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1097\/01.shk.0000151028.15377.f7"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1038\/35021228"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.279.2.H791"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-200013020-00008"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-200201001-00010"},{"key":"R6","doi-asserted-by":"crossref","unstructured":"Ayala A, Perrin MM, Wang P, Ertel W, and Chaudry IH.Hemorrhage induces enhanced Kupffer cell cytotoxicity while decreasing peritoneal or splenic macrophage capacity. Involvement of cell-associated tumor necrosis factor and reactive nitrogen.J Immunol147: 4147\u20134154, 1991.","DOI":"10.4049\/jimmunol.147.12.4147"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199706000-00021"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-199808000-00001"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.2174\/1381612043384420"},{"key":"R10","doi-asserted-by":"crossref","unstructured":"Bolke E, Jehle PM, Hausmann F, Daubler A, Wiedeck H, Steinbach G, Storck M, and Orth K.Preoperative oral application of immunoglobulin-enriched colostrum milk and mediator response during abdominal surgery.Shock17: 9\u201312, 2002.","DOI":"10.1097\/00024382-200201000-00002"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204105"},{"key":"R12","unstructured":"Deitch EA, Bridges W, Baker J, Ma JW, Ma L, Grisham MB, Granger DN, Specian RD, and Berg R.Hemorrhagic shock-induced bacterial translocation is reduced by xanthine oxidase inhibition or inactivation.Surgery104: 191\u2013198, 1988."},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1097\/00005373-199904000-00018"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-200209000-00008"},{"key":"R15","unstructured":"Feng J, Liu Y, Shi J, and Zhang R.[The role of LPS in the CD14 expression and the activation of Kupffer cells].Zhonghua Shao Shang Za Zhi18: 107\u2013111, 2002."},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.11.6942-6950.2001"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1097\/00005373-199809000-00022"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.6.917"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000041221.13644.B9"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-200115050-00012"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-200014050-00010"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1038\/39622"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(02)00867-5"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00465.2001"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-200003000-00013"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-199703000-00009"},{"key":"R27","doi-asserted-by":"crossref","unstructured":"Koziol JM, Rush BF Jr, Smith SM, and Machiedo GW.Occurrence of bacteremia during and after hemorrhagic shock.J Trauma28: 10\u201316, 1988.","DOI":"10.1097\/00005373-198801000-00002"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.57.3.440"},{"key":"R29","doi-asserted-by":"crossref","unstructured":"Le Roy D, Di Padova F, Tees R, Lengacher S, Landmann R, Glauser MP, Calandra T, and Heumann D.Monoclonal antibodies to murine lipopolysaccharide (LPS)-binding protein (LBP) protect mice from lethal endotoxemia by blocking either the binding of LPS to LBP or the presentation of LPS\/LBP complexes to CD14.J Immunol162: 7454\u20137460, 1999.","DOI":"10.4049\/jimmunol.162.12.7454"},{"key":"R30","doi-asserted-by":"crossref","unstructured":"Lehnert M, Uehara T, Bradford BU, Lind H, Paik YH, Zhong Z, Brenner DA, Marzi I, and Lemasters JJ.Lipopolysaccharide-binding protein modulates hepatic damage and inflammatory response after hemorrhagic shock and resuscitation.Shock19,Suppl: 28, 2005.","DOI":"10.1097\/00024382-200306001-00081"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-200304000-00009"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.37.1.327"},{"key":"R33","doi-asserted-by":"crossref","unstructured":"Mathison JC, Tobias PS, Wolfson E, and Ulevitch RJ.Plasma lipopolysaccharide (LPS)-binding protein. A key component in macrophage recognition of gram-negative LPS.J Immunol149: 200\u2013206, 1992.","DOI":"10.4049\/jimmunol.149.1.200"},{"key":"R34","doi-asserted-by":"crossref","unstructured":"Meng ZH, Dyer K, Billiar TR, and Tweardy DJ.Essential role for IL-6 in postresuscitation inflammation in hemorrhagic shock.Am J Physiol Cell Physiol280: C343\u2013C351, 2001.","DOI":"10.1152\/ajpcell.2001.280.2.C343"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-200116060-00010"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1097\/00005373-199105000-00006"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100199200"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-200110000-00019"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1998.tb05933.x"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1016\/0741-5214(93)90341-I"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-198805000-00009"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1126\/science.2402637"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-200302000-00014"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1053\/he.2000.5634"},{"key":"R45","doi-asserted-by":"crossref","unstructured":"Uehara T, Xi P, X, Bennett B, Satoh Y, Friedman G, Currin R, Brenner DA, and Lemasters J.c-Jun N-terminal kinase mediates hepatic injury after rat liver transplantation.Transplantation78: 324\u2013332, 2004.","DOI":"10.1097\/01.TP.0000128859.42696.28"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.6.2963"},{"key":"R47","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.1998.5290"},{"key":"R48","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.3.1025"},{"key":"R49","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.12.2051"},{"key":"R50","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-199504000-00011"},{"key":"R51","doi-asserted-by":"crossref","unstructured":"Zhong Z, Enomoto N, Connor HD, Moss N, Mason RP, and Thurman RG.Glycine improves survival after hemorrhagic shock in the rat.Shock12: 54\u201362, 1999.","DOI":"10.1097\/00024382-199907000-00008"},{"key":"R52","doi-asserted-by":"publisher","DOI":"10.1177\/0148607103027004241"}],"container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.00480.2005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,7]],"date-time":"2023-05-07T02:21:10Z","timestamp":1683426070000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.00480.2005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9]]},"references-count":52,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,9]]}},"alternative-id":["10.1152\/ajpgi.00480.2005"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.00480.2005","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,9]]}}}