{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T22:19:37Z","timestamp":1773958777187,"version":"3.50.1"},"reference-count":46,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:p> Using whole cell patch clamp in thin brain stem slices, we tested the effects of cholecystokinin (CCK) on identified gastric-projecting neurons of the rat dorsal motor nucleus of the vagus (DMV). Perfusion with the sulfated form of CCK octapeptide (CCK8s, 30 pM\u2013300 nM, EC<jats:sub>50<\/jats:sub> \u223c4 nM) induced a concentration-dependent inward current in 35 and 41% of corpus- and antrum\/pylorus-projecting DMV neurons, respectively. Conversely, none of the fundus-projecting DMV neurons responded to perfusion with CCK8s. The CCK8s-induced inward current was accompanied by a 65 \u00b1 17% increase in membrane input resistance and reversed at 90 \u00b1 4 mV, indicating that the excitatory effects of CCK8s were mediated by the closure of a potassium conductance. Pretreatment with the synaptic blocker TTX (0.3\u20131 \u03bcM) reduced the CCK8s-induced current, suggesting that a portion of the CCK8s-induced current was mediated indirectly via an action on presynaptic neurons apposing the DMV membrane. Pretreatment with the selective CCK-A receptor antagonist lorglumide (0.3\u20133 \u03bcM) attenuated the CCK8s-induced inward current in a concentration-dependent manner, with a maximum inhibition of 69 \u00b1 12% obtained with 3 \u03bcM lorglumide. Conversely, pretreatment with the selective CCK-B antagonist triglumide did not attenuate the CCK8s-induced inward current; pretreatment with triglumide (3 \u03bcM) and lorglumide (1 \u03bcM) attenuated the CCK8s-induced current to the same extent as pretreatment with lorglumide alone. Immunohistochemical experiments showed that CCK-A receptors were localized on the membrane of 34, 65, and 60% of fundus-, corpus-, and antrum\/pylorus-projecting DMV neurons, respectively. Our data indicate that CCK-A receptors are present on a subpopulation of gastric-projecting neurons and that their activation leads to excitation of the DMV membrane. <\/jats:p>","DOI":"10.1152\/ajpgi.00497.2004","type":"journal-article","created":{"date-parts":[[2004,12,10]],"date-time":"2004-12-10T01:25:08Z","timestamp":1102641908000},"page":"G1066-G1073","source":"Crossref","is-referenced-by-count":28,"title":["In vitro analysis of the effects of cholecystokinin on rat brain stem motoneurons"],"prefix":"10.1152","volume":"288","author":[{"given":"Zhongling","family":"Zheng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark W.","family":"Lewis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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