{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T08:56:04Z","timestamp":1769072164544,"version":"3.49.0"},"reference-count":38,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:p>The mechanosensitive endings of low-threshold, slowly adapting pelvic afferents that innervate the rectum have been previously identified as rectal intraganglionic laminar endings (rIGLEs) that lie within myenteric ganglia. We tested whether the aganglionic rectum of piebald-lethal (s<jats:sup>l<\/jats:sup>\/s<jats:sup>l<\/jats:sup>) mice lacks rIGLEs and whether this could explain impaired distension-evoked reflexes from this region. Extracellular recordings were made from fine rectal nerves in C57BL\/6 wild-type and s<jats:sup>l<\/jats:sup>\/s<jats:sup>l<\/jats:sup>mice, combined with anterograde labeling. In C57BL\/6 mice, graded circumferential stretch applied to the rectum activated graded increases in firing of slowly adapting rectal mechanoreceptors. In s<jats:sup>l<\/jats:sup>\/s<jats:sup>l<\/jats:sup>mice, graded stretch of the aganglionic rectum activated similar graded increases in rectal afferent firing. Stretch-sensitive afferents responded at low mechanical thresholds and fired more intensely at noxious levels of stretch. They could also be activated by probing their receptive fields with von Frey hairs and by muscle contraction. Anterograde labeling from recorded rectal nerves identified the mechanoreceptors of muscular afferents in the aganglionic rectal smooth muscle. A population of afferents were also recorded in both C57BL\/6 and s<jats:sup>l<\/jats:sup>\/s<jats:sup>l<\/jats:sup>mice that were activated by von Frey hair probing, but not stretch. In summary, the aganglionic rectum is innervated by a population of stretch-sensitive rectal afferent mechanoreceptor which develops and functions in the absence of any enteric ganglia. These results suggest that in patients with Hirschsprung's disease the inability to activate extrinsic distension reflexes from the aganglionic rectum is unlikely to be due to the absence of stretch-sensitive extrinsic mechanoreceptors.<\/jats:p>","DOI":"10.1152\/ajpgi.00502.2007","type":"journal-article","created":{"date-parts":[[2008,1,25]],"date-time":"2008-01-25T01:34:17Z","timestamp":1201224857000},"page":"G855-G867","source":"Crossref","is-referenced-by-count":32,"title":["Identification of functional intramuscular rectal mechanoreceptors in aganglionic rectal smooth muscle from piebald lethal mice"],"prefix":"10.1152","volume":"294","author":[{"given":"Nick J.","family":"Spencer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aoife","family":"Kerrin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir P.","family":"Zagorodnyuk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grant W.","family":"Hennig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melodie","family":"Muto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon J.","family":"Brookes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orla","family":"McDonnell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1838(90)90185-L"},{"key":"R2","doi-asserted-by":"crossref","unstructured":"Blackshaw LA, Brookes SJ, Grundy D, Schemann M.Sensory transmission in the gastrointestinal tract.Neurogastroenterol Motil19: 1\u201319, 2001.","DOI":"10.1111\/j.1365-2982.2006.00871.x"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1007\/BF01071956"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.089714"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.04.008"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0525v.x"},{"key":"R7","doi-asserted-by":"crossref","unstructured":"Gariepy CE, Cass DT, Yanagisawa M.Null mutation of endothelin receptor type B gene in spotting lethal rats causes aganglionic megacolon and white coat color.Proc Natl Acad Sci USA23: 867\u2013872, 1996.","DOI":"10.1073\/pnas.93.2.867"},{"key":"R8","unstructured":"Garrett JR, Howard ER.Myenteric plexus of the hind-gut: developmental abnormalities in humans and experimental studies.Ciba Found Symp83: 326\u2013354, 1981."},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1994.266.6.G1139"},{"key":"R10","unstructured":"Grundy D, Scratcherd T.Sensory afferents from the gastrointestinal tract. 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