{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:38:41Z","timestamp":1792096721965,"version":"4.4.0"},"reference-count":33,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:p>Organic solute transporter (OST\u03b1-OST\u03b2) is a novel heteromeric bile acid and sterol transporter expressed at the basolateral membranes of epithelium in the ileum, kidney, and liver. To determine whether OST\u03b1-OST\u03b2 undergoes farnesoid X receptor (FXR)-dependent adaptive regulation following cholestatic liver injury, mRNA and protein expression levels were analyzed in patients with primary biliary cirrhosis (PBC) and following common bile duct ligation (CBDL) in rats and Fxr null and wild-type mice. Hepatic OST\u03b1 and OST\u03b2 mRNA increased 3- and 32-fold, respectively, in patients with PBC compared with controls, whereas expression of Ost\u03b1 and Ost\u03b2 also increased in the liver of rats and mice following CBDL. In contrast, expression of Ost\u03b1 and Ost\u03b2 mRNA was generally lower in Fxr null mice, and CBDL failed to enhance expression of Ost\u03b1 and Ost\u03b2 compared with wild-type mice. HepG2 cells treated for 24 h with chenodeoxycholic acid, a selective FXR ligand, had higher levels of OST\u03b1 and OST\u03b2 mRNA and protein. Increases in OST protein were visualized by confocal microscopy at the plasma membrane. These results indicate that expression of Ost\u03b1 and Ost\u03b2 are highly regulated in response to cholestasis and that this response is dependent on the FXR bile acid receptor.<\/jats:p>","DOI":"10.1152\/ajpgi.00539.2005","type":"journal-article","created":{"date-parts":[[2006,1,19]],"date-time":"2006-01-19T20:40:19Z","timestamp":1137703219000},"page":"G1124-G1130","source":"Crossref","is-referenced-by-count":266,"title":["Upregulation of a basolateral FXR-dependent bile acid efflux transporter OST\u03b1-OST\u03b2 in cholestasis in humans and rodents"],"prefix":"10.1152","volume":"290","author":[{"given":"James 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