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Cells were isolated from longitudinal muscle myenteric plexus (LMMP). Cytokine receptor expression, muscle contractility, and muscarinic agonist receptor characteristics were examined by agonist displacement of [ N- methyl-<jats:sup>3<\/jats:sup>H]scopolamine ([<jats:sup>3<\/jats:sup>H]NMS) binding. The TGF-\u03b21 receptor (543 bp) and the IFN-\u03b3 receptor 1 (660 bp) were identified on smooth muscle cells. Scatchard analysis revealed dissociation constant and maximum binding values for [<jats:sup>3<\/jats:sup>H]NMS of 2.6 nM and 2.4 \u00d7 10<jats:sup>4<\/jats:sup>sites\/cell, respectively, in control cells. Nematode infection was accompanied by a reduction in inhibitory constant of the high-affinity sites ( K<jats:sub>H<\/jats:sub>), and this was independent of signal transduction and activator of transcription 6. Preincubation with TGF-\u03b21 enhanced longitudinal muscle contractility and decreased the K<jats:sub>H<\/jats:sub>to 2.2 pM (increased muscarinic receptor affinity), whereas preincubation with IFN-\u03b3 increased the K<jats:sub>H<\/jats:sub>to 0.4 \u03bcM (decreased muscarinic receptor affinity) and decreased longitudinal muscle contractility. Preincubation of LMMP with IL-13 decreased the K<jats:sub>H<\/jats:sub>to 0.2 nM. Cytokines exert differential effects on the muscarinic receptor on intestinal longitudinal smooth muscle. These findings explain the basis for altered muscle contractility observed in Th1 and Th2 models of inflammation, as well as in the post-nematode-infected state.<\/jats:p>","DOI":"10.1152\/ajpgi.00545.2006","type":"journal-article","created":{"date-parts":[[2007,4,13]],"date-time":"2007-04-13T00:53:42Z","timestamp":1176425622000},"page":"G250-G255","source":"Crossref","is-referenced-by-count":25,"title":["Cytokine modulation of muscarinic receptors in the murine intestine"],"prefix":"10.1152","volume":"293","author":[{"given":"Hirotada","family":"Akiho","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Waliul 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