{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T19:58:50Z","timestamp":1722023930846},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1982,4,1]]},"abstract":"<jats:p> Weanling female 129\/J mice were maintained for 1, 2, 3, or 6 mo on either a control diet containing 60% sucrose and 23% protein or an isocaloric, high-protein, no-carbohydrate diet containing 83% protein and 0% sucrose. Mice were killed after each interval to assess the effect of diet on histological and physiological changes in the endocrine pancreas. Image analysis of islets stained immunocytochemically for alpha-cells, beta-cells, delta-cells, and PP cells was performed to quantify changes in islet structure. It was found that islet composition was strongly affected by diet. The volume density of the alpha-cells was significantly elevated in mice fed the high-protein diet (e.g., 35% vs. 16% in controls at 6 mo), whereas the volume density of beta-cells concomitantly decreased from 65 to 39%. Radioimmunoassay of the insulin and glucagon content of the pancreas and the plasma corroborated the morphometric findings. Pancreatic and plasma glucagon concentration in mice on the high-protein diet was elevated by an average of 2.5-fold above controls, whereas pancreatic insulin concentration was diminished by nearly half. The increase in alpha-cell volume density and pancreatic glucagon concentration appeared initially due to alpha-cell hypertrophy, although by 6 mo of high-protein feeding both hypertrophy and hyperplasia of the alpha-cells were evident. Presumably, these changes were compensatory responses to the increased functional demand on alpha-cells (i.e., glucagon biosynthesis and secretion) imposed by chronic high-protein feeding. <\/jats:p>","DOI":"10.1152\/ajpgi.1982.242.4.g354","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T23:32:17Z","timestamp":1514071937000},"page":"G354-G359","source":"Crossref","is-referenced-by-count":3,"title":["Dietary modulation of alpha-cell volume and function in strain 129\/J mice"],"prefix":"10.1152","volume":"242","author":[{"given":"M. G.","family":"Morley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. H.","family":"Leiter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. B.","family":"Eisenstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Strack","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1982.242.4.G354","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T22:13:09Z","timestamp":1567980789000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1982.242.4.G354"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1982,4,1]]}},"alternative-id":["10.1152\/ajpgi.1982.242.4.G354"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1982.242.4.g354","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,4,1]]}}}