{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T12:42:34Z","timestamp":1649162554461},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1985,3,1]]},"abstract":"<jats:p> Intraluminal pressures and myoelectric activity were recorded from the feline antrum, pylorus, and duodenum in response to intraduodenal amino acid solutions. Mixed amino acids (0.02 mg\/ml, 3.0 ml) increased the amplitude of pyloric contractions (59.7 +\/- 7.9 mmHg) and pyloric spike activity (73.7 +\/- 6.8% of slow waves with spike activity) compared with a saline control (P less than 0.001). The selectivity of these responses was determined with specific amino acids. L-Tryptophan (10 or 40 mM) produced a response similar to the mixed amino acid response, while L-phenylalanine or L-glycine (10 or 40 mM) had no effect. Intra-arterial tetrodotoxin, intraluminal ethyl aminobenzoate, or intravenous naloxone (1.0 mg\/kg) abolished the pyloric responses to amino acids (P less than 0.02). Bilateral cervical vagotomy had no effect. Cholecystokinin octapeptide (CCK-OP) produced dose-dependent increases in the amplitude of pyloric contractions and in pyloric spike activity. The ED50 dose of CCK-OP (1.0 microgram\/kg iv) gave an increase in pyloric pressure of 155.6 +\/- 49.9 mmHg and in spike activity of 77.7 +\/- 9.4%, similar to mixed amino acids or tryptophan. These effects of CCK-OP were not antagonized, however, by a dose of naloxone (1.0 mg\/kg) that blocked the maximal pyloric response to leucine-enkephalin. We concluded intraduodenal mixed amino acids or tryptophan increase phasic, spike-dependent pyloric contractions in the cat via nonvagal, naloxone-sensitive neural pathways, phenylalanine, a structurally similar essential amino acid, had no effect on the feline gastroduodenal junction, and the pyloric responses to exogenous CCK-OP are mediated by pathways distinct from the responses to tryptophan or mixed amino acids. <\/jats:p>","DOI":"10.1152\/ajpgi.1985.248.3.g307","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T20:00:22Z","timestamp":1514059222000},"page":"G307-G312","source":"Crossref","is-referenced-by-count":1,"title":["Opiate nerves mediate feline pyloric response to intraduodenal amino acids"],"prefix":"10.1152","volume":"248","author":[{"given":"J. C.","family":"Reynolds","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Ouyang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1985.248.3.G307","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T21:38:47Z","timestamp":1567978727000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1985.248.3.G307"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1985,3,1]]}},"alternative-id":["10.1152\/ajpgi.1985.248.3.G307"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1985.248.3.g307","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,3,1]]}}}