{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T17:14:11Z","timestamp":1649092451767},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1989,7,1]]},"abstract":"<jats:p> The actions of three different types of calcium channel blockers on short-circuit current (Isc) in rabbit ileum were studied. These included the phenylalkylamines, verapamil and (l)-desmethoxyverapamil (D888); the dihydropyridines, nifedipine and nitrendipine; and the benzothiazepine, diltiazem. All of the drugs decreased Isc, a change associated with increased Na and Cl absorption. Verapamil and D888 had the largest effects. The dihydropyridine, BAY K 8644, a calcium channel activator, increased Isc and decreased Na and Cl absorption, effects not inhibited by tetrodotoxin. The phenylalkylamines had an additional effect on Isc in the presence of a maximally inhibitory concentration of the dihydropyridines, suggesting the possibility of two distinct calcium channels, one of which is the L-type voltage-activated, dihydropyridine- and phenylalkylamine-sensitive channel, and the other is a channel only sensitive to phenylalkylamines but not to dihydropyridines. [3H]nitrendipine and [3H]D888 binding to an enriched preparation of basolateral membranes from ileal epithelial cells was characterized. Each ligand bound specifically and saturably to an apparently single population of high-affinity sites with [3H]D888 having three times as many binding sites as [3H]nitrendipine. [3H]nitrendipine binding was partially inhibited by verapamil and D888 and was increased by diltiazem; whereas [3H]D888 binding was inhibited completely by verapamil but only partially by nitrendipine and diltiazem. These transport and binding studies suggest the presence of two types of Ca2+ channels in ileal epithelial cells, one of which interacts with the dihydropyridines, the phenylalkylamines, and the benzothiazepines at three different sites and the other channel that only binds the phenylalkylamines. <\/jats:p>","DOI":"10.1152\/ajpgi.1989.257.1.g86","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T02:34:29Z","timestamp":1514082869000},"page":"G86-G93","source":"Crossref","is-referenced-by-count":0,"title":["Two calcium channels in basolateral membranes of rabbit ileal epithelial cells"],"prefix":"10.1152","volume":"257","author":[{"given":"F. R.","family":"Homaidan","sequence":"first","affiliation":[{"name":"Department of Pharmacology, New York State College of VeterinaryMedicine, Cornell University, Ithaca 14853."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Donowitz","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, New York State College of VeterinaryMedicine, Cornell University, Ithaca 14853."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. A.","family":"Weiland","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, New York State College of VeterinaryMedicine, Cornell University, Ithaca 14853."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. W.","family":"Sharp","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, New York State College of VeterinaryMedicine, Cornell University, Ithaca 14853."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1989.257.1.G86","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T19:23:59Z","timestamp":1567970639000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1989.257.1.G86"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1989,7,1]]}},"alternative-id":["10.1152\/ajpgi.1989.257.1.G86"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1989.257.1.g86","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,7,1]]}}}