{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T02:19:28Z","timestamp":1720664368568},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1990,12,1]]},"abstract":"<jats:p> Milk from lactating Sprague-Dawley rats was assayed for epidermal growth factor (EGF)-like activities. A homologous radioimmunoassay (RIA) indicated the presence of immunoreactive material that competed in a nonparallel fashion with submandibular gland rat EGF (sm-r-EGF). The activity in the milk was extracted using antibodies to sm-r-EGF covalently linked to acrylamide beads. This activity was characterized by RIA, nondenaturing polyacrylamide gel electrophoresis, enzymatic digestion, radioreceptor assay, and ability to stimulate incorporation of [3H]thymidine into cultured fibroblasts. The presence of three distinct immunoreactive forms of EGF in rat milk were detected that competed with 125I-labeled r-EGF for binding to the EGF receptor and stimulated DNA synthesis in growth-arrested fibroblasts. Two of the forms are converted to the sm-r-EGF species by tryptic digestion as determined by RIA and migration rates in a nondenaturing polyacrylamide gel. The biological activities are stable to heating in 0.1 M acetic acid and also are stable at pH 9.0. Levels of r-EGF equivalents in milk were low at time of birth (6.3 +\/- 1.7 ng\/ml) and rose to 35.4 +\/- 14.6 by days 4 to 6. <\/jats:p>","DOI":"10.1152\/ajpgi.1990.259.6.g1056","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T07:56:37Z","timestamp":1514102197000},"page":"G1056-G1061","source":"Crossref","is-referenced-by-count":1,"title":["Identification and partial characterization of multiple forms of biologically active EGF in rat milk"],"prefix":"10.1152","volume":"259","author":[{"given":"R. P.","family":"Schaudies","sequence":"first","affiliation":[{"name":"Department of Clinical Investigation, Walter Reed Army Medical Center,Washington, DC 20307."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Grimes","sequence":"additional","affiliation":[{"name":"Department of Clinical Investigation, Walter Reed Army Medical Center,Washington, DC 20307."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. L.","family":"Wray","sequence":"additional","affiliation":[{"name":"Department of Clinical Investigation, Walter Reed Army Medical Center,Washington, DC 20307."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Koldovsky","sequence":"additional","affiliation":[{"name":"Department of Clinical Investigation, Walter Reed Army Medical Center,Washington, DC 20307."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1990.259.6.G1056","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T23:28:55Z","timestamp":1567985335000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1990.259.6.G1056"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1990,12,1]]}},"alternative-id":["10.1152\/ajpgi.1990.259.6.G1056"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1990.259.6.g1056","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,12,1]]}}}