{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,27]],"date-time":"2024-07-27T02:14:25Z","timestamp":1722046465160},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1992,3,1]]},"abstract":"<jats:p> Enteropancreatic 5-hydroxytryptamine (5-HT)-immunoreactive nerve fibers have recently been described. To test the hypothesis that pancreas contains 5-HT receptors similar to those that characterize the enteric nervous system, binding of 5-[3H]HT to pancreatic membranes was investigated. Membranes were prepared by differential centrifugation from guinea pig pancreas, and binding of 5-[3H]HT was measured by use of a rapid filtration assay. Alternatively, 5-[3H]HT-binding sites were analyzed and located by radioautography on frozen sections of unfixed pancreas. The 5-HT1P receptor-specific antagonist, N-acetyl-5-hydroxytryptophyl-5-hydroxytryptophan amide (5-HTP-DP), was used to define nonspecific binding in all assays. High-affinity reversible binding of 5-[3H]HT to pancreatic membranes was found. Good agreement was observed when Kd was determined from saturation isotherms (Kd = 2.1 +\/- 0.4 nM; Bmax = 0.6 +\/- 0.07 pmol\/mg protein) or from the ratio of association and dissociation constants (2.7 +\/- 0.04 nM). As is true of 5-[3H]HT binding to enteric 5-HT1P receptors, binding of 5-[3H]HT to pancreatic membranes was inhibited by 5-HTP-DP, 5- and 6-hydroxyindalpine (agonists at 5-HT1P receptors), and guanine nucleotides. In contrast, binding of 5-[3H]HT to pancreatic membranes was not inhibited by a variety of compounds known to bind to other types of 5-HT receptor, to the neuronal 5-HT transporter, to alpha- or beta-adrenoceptors, or to dopamine receptors. We concluded that pancreas contains 5-HT1P receptors with properties similar to those of bowel. Radioautography and immunocytochemistry with an anti-idiotypic antibody that recognizes 5-HT receptors revealed that pancreatic 5-HT1P receptors (identified with appropriate inhibitors of 5-[3H]HT or antibody binding) were concentrated in ganglia, nerves, and unidentified cells in both exocrine and endocrine pancreas. These observations suggest that future studies of 5-HT's role in pancreatic physiology should concentrate on enteropancreatic innervation and 5-HT1P receptors. <\/jats:p>","DOI":"10.1152\/ajpgi.1992.262.3.g553","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T21:46:32Z","timestamp":1514065592000},"page":"G553-G566","source":"Crossref","is-referenced-by-count":3,"title":["Identification and localization of 5-HT1P receptors in the guinea pig pancreas"],"prefix":"10.1152","volume":"262","author":[{"given":"A. L.","family":"Kirchgessner","sequence":"first","affiliation":[{"name":"Department of Anatomy and Cell Biology, Columbia University College ofPhysicians and Surgeons, New York, New York 10032."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. T.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, Columbia University College ofPhysicians and Surgeons, New York, New York 10032."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Tamir","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, Columbia University College ofPhysicians and Surgeons, New York, New York 10032."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. D.","family":"Gershon","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, Columbia University College ofPhysicians and Surgeons, New York, New York 10032."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1992.262.3.G553","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T18:45:22Z","timestamp":1567968322000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1992.262.3.G553"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1992,3,1]]}},"alternative-id":["10.1152\/ajpgi.1992.262.3.G553"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1992.262.3.g553","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,3,1]]}}}